ARTICLE: PDF OnlyCarlson William D.; Handschumacher, Mark; Summers, Neena; Karplus, Martin; Haber, EdgarJournal of Cardiovascular Pharmacology: 1987 - p 91-93 Free Abstract Summary Three-dimensional models of the octapeptide segment of renin substrate and of several inhibitors characterized by a modified scissile bond were constructed at the active site of human renin. The substrate and inhibitor models were based on the structure of pepstatin solved by x-ray crystallography to a resolution of 1.8 À. The renin structure, previously reported by us, was based on the structures of homologous aspartyl proteases solved by x-ray diffraction techniques to a resolution of 2.1 A or higher. An energy minimization program, CHARMM, was used to refine these structures with respect to the optimal interaction of the enzyme and its inhibitor, and to calculate the energy of each inhibitor-enzyme interaction. The results indicate that the most significant difference between the binding of inhibitors and of the natural substrate is caused by a reduction in electrostatic rather than conformational strain. Copyright © 1987 Wolters Kluwer Health, Inc. All rights reserved.