ORIGINAL ARTICLESThe efficacy of Ankaferd Blood Stopper in antithrombotic drug-induced primary and secondary hemostatic abnormalities of a rat-bleeding modelKosar, Alia; Cipil, Handan Sa; Kaya, Arifa; Uz, Buraka; Haznedaroglu, Ibrahim Cb; Goker, Hakanb; Ozdemir, Oktayc; Ercetin, Sevild; Kirazli, Serafettind; Firat, Huseyin Cahitd Author Information aDepartments of Hematology and Surgery, Faculty of Medicine, University of Fatih, Turkey bDepartment of Hematology, Faculty of Medicine, University of Hacettepe, Ankara, Turkey cDepartment of Scientific Research, Yorum Konsultancy Inc., Turkey dDepartment of Research and Development, Ankaferd Drug Inc., Istanbul, Turkey Received 18 May, 2008 Revised 24 September, 2008 Accepted 5 October, 2008 Correspondence to Ibrahim C. Haznedaroglu, MD, Department of Hematology, Hacettepe University Medical School, Sihhiye, Ankara TR-06100, Turkey E-mail: [email protected] Blood Coagulation & Fibrinolysis 20(3):p 185-190, April 2009. | DOI: 10.1097/MBC.0b013e32831c4cb0 Buy Metrics Abstract Ankaferd comprises a standardized mixture of plants Thymus vulgaris, Glycyrrhiza glabra, Vitis vinifera, Alpinia officinarum and Urtica dioica. Ankaferd Blood Stopper (ABS) as a medicinal product has been approved in the management of external hemorrhage and dental surgery bleedings in Turkey. This study aimed to evaluate the in-vivo hemostatic effect of ABS in rats pretreated with acetylsalicylic acid or enoxaparin. Wistar rats (210–270 g) of both sexes were used in this study. The animals were pretreated with acetylsalicylic acid (10 mg/kg) orally for 4 days or enoxaparin sodium (8 mg/kg) subcutaneously for 3 days or did not receive any anticoagulant before tail cut at 4th day. ABS was administered topically [a total of 4 ml (1 ml/puff × 4)] to the cut tail in the studied animals. The duration of bleeding and the amount of bleeding were measured in order to evaluate the hemostatic effect of ABS. In acetylsalicylic acid-treated animals, topical ABS reduced both the duration and also the amount of bleeding volume by 68.4 and 54.6%, respectively. It was also effective in shortening the duration of bleeding (30.6%) and decreasing the amount of bleeding (32.8%) in enoxaparin-treated animals. ABS, a traditional folkloric medicinal plant extract, has in-vivo hemostatic actions, which may provide a therapeutic potential for the management of patients with deficient hemostasis in the clinical medicine. © 2009 Lippincott Williams & Wilkins, Inc.