ORIGINAL ARTICLESD-Dimer and prothrombin fragment 1 + 2 in urine and plasma in patients with clinically suspected venous thromboembolismWexels, Fredrik; Seljeflot, Ingebjørg; Pripp, Are H.; Dahl, Ola E.Author Information aDepartment of Radiology, Vestre Viken Hospital Trust, Drammen bFaculty of Medicine, University of Oslo cDepartment of Cardiology, Centre for Clinical Heart Research, Oslo University Hospital dOslo Centre of Biostatistics and Epidemiology, Research Support Services, Oslo University Hospital, Oslo eCentre of Medical Science, Education and Innovation, Innlandet Hospital Trust, Brumunddal, Norway fThrombosis Research Institute, London, UK Correspondence to Fredrik Wexels, Department of Radiology, Vestre Viken Hospital Trust, 3004 Drammen, Norway Tel: +47 32 80 37 02; e-mail: [email protected] Received 21 April, 2015 Revised 10 September, 2015 Accepted 26 September, 2015 Blood Coagulation & Fibrinolysis: June 2016 - Volume 27 - Issue 4 - p 396-400 doi: 10.1097/MBC.0000000000000461 Buy Metrics Abstract Increased levels of urine prothrombin fragment 1 + 2 was recently reported to be associated with imaging-verified venous thromboembolism. In this study we evaluated the relationship between plasma D-dimer and plasma and urine prothrombin fragment 1 + 2 in patients with suspected venous thromboembolism. Urine and blood samples were collected from patients with suspected pulmonary embolism or deep vein thrombosis. The samples were analysed with commercially available ELISA kits. The diagnosis of venous thromboembolism was verified with contrast-enhanced computer tomography of the pulmonary arteries or lower extremity deep vein compression ultrasound and venography as appropriate. Venous thromboembolism was diagnosed in 150 of 720 patients. Significantly higher levels of plasma D-dimer and prothrombin fragment 1 + 2 in plasma and urine were found in those with imaging-confirmed venous thromboembolism versus those without (P < 0.001). The correlation between the three biomarkers was statistically significant (range of rs values 0.45–0.65, P < 0.001). Plasma D-dimer had the highest diagnostic accuracy followed by prothrombin fragment 1 + 2 in plasma. Further development of ELISA analyses for urine testing of prothrombin fragment 1 + 2 may improve its diagnostic accuracy. Copyright © 2016 YEAR Wolters Kluwer Health, Inc. All rights reserved.