TECHNICAL REPORTSIn-vitro model for the ultrastructural study of the formation of thrombi in human plateletsCerecedo, Dorisa,c; González, Sireniab; Mondragón, Mónicaa; Reyes, Elbac; Mondragón, RicardoaAuthor Information aDepartment of Biochemistry bElectron Microscopy Facility, Center of Research and Advanced Studies (CINVESTAV) cDepartment of Morphology, National School of Biological Sciences, Mexico City, Mexico Received 6 October, 2005 Accepted 8 December, 2005 Correspondence and requests for reprints to Dr Ricardo Mondragón, Department Bioquímica, Centro de Investigación y Estudios Avanzados, Av. IPN #2508, Col. Zacatenco, Del. G.A. Madero, México D.F. C.P. 07360, Mexico Tel: +52 55 50 61 39 49; fax: +52 55 50 61 33 91; e-mail: [email protected]; [email protected] Sponsorship: This work was supported by research grants CONACyT 37713-N (R.M.). Blood Coagulation & Fibrinolysis: March 2006 - Volume 17 - Issue 2 - p 161-164 doi: 10.1097/01.mbc.0000214712.96244.47 Buy Metrics Abstract Platelets are cell fragments with dynamic properties involved in clot formation after tissue damage. Platelet activation causes a change in shape, secretion of intracellular granules and aggregation with each other through the cytoskeleton components and biochemical changes. Platelet adhesion, considered as the major event in haemostasis, has been studied in several in-vitro and in-vivo models to evaluate the feasible thrombogenicity of some materials, the dynamics of specific receptors, as well as the effect of different buffers and inhibitors in this process. In spite of the numerous reports about platelet activation, to date there is no information available about the fine structure of the platelet–platelet and platelet–substrate interactions. In the present report we describe an in-vitro system that allows the visualization of these interactions: platelets are adhered to an inert substrate, and interactions with suspended platelets as a process to initiate the formation of thrombi was followed by ultramicrotomy and transmission electron microscopy. © 2006 Lippincott Williams & Wilkins, Inc.