TECHNICAL NOTEMulti-centre investigation on reference ranges for ROTEM thromboelastometryLang, Thomasa; Bauters, Anneb; Braun, Siegmund Lc; Pötzsch, Berndd; von Pape, Klaus-Wernere; Kolde, Hans-Jürgenf; Lakner, MeretfAuthor Information aClinical Institute of Medical and Chemical Laboratory Diagnostic, University Clinic of Graz, Austria bInstitut d’Hématologie Biologique, ESPRI-EA 2693, University Hospital of Lille, France cInstitute of Laboratory Medicine, German Heart Centre Munich dInstitute for Experimental Haematology and Transfusion Medicine, University of Bonn eInstitute of Laboratory Medicine, Hospital Fulda fPentapharm GmbH, Diagnostic Division, Munich, Germany Received 23 July, 2004 Revised 9 December, 2004 Accepted 10 December, 2004 Correspondence and requests for reprints to Meret Lakner, Pentapharm GmbH, Diagnostic Division, Stahlgruberring 12, 81829 München, Germany. Tel: +49 89 4542 9516; fax: +49 89 4542 9522; e-mail: [email protected] Blood Coagulation & Fibrinolysis: June 2005 - Volume 16 - Issue 4 - p 301-310 doi: 10.1097/01.mbc.0000169225.31173.19 Buy Metrics Abstract Reagent-supported thromboelastometry (TEM) with the ROTEM Whole Blood Haemostasis Analyser is an enhancement of thromboelastography, a method that is increasingly used for the point of care monitoring of acute perioperative bleeding disorders. We investigated the reference ranges of two activated tests (INTEM and EXTEM) and a test analysing specifically the fibrin component of coagulation (FIBTEM) in a multi-centre approach. The reference ranges obtained for the clotting time (CT), clot formation time (CFT), alpha angle (ALP), maximum clot firmness (MCF) and clot lysis parameters were comparable from centre to centre. INTEM: CT equals; 137–246 s, CFT equals; 40–100 s, MCF equals; 52–72 mm. EXTEM: CT equals; 42–74 s, CFT equals; 46–148 s, MCF equals; 49–71 mm. FIBTEM: MCF equals; 9–25 mm. ROTEM whole blood coagulation correlated weakly with a trend towards enhanced coagulation in females compared with males and in advanced age. The repeatability (within-run imprecision) of the results was dependent on the test with the following coefficients of variation: 1–5% (clot firmness, alpha angle), 3–12% (CT, CFT), 6–13% (FIBTEM clot firmness). Citrated blood samples were stable for ROTEM analysis stored within 6 h from drawing. In summary, the data showed that ROTEM thromboelastometry yields consistent values between centres and that providing general orientating reference ranges seems to be possible. © 2005 Lippincott Williams & Wilkins, Inc.