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Stability of coagulation proteins in frozen plasma

Woodhams, B.; Girardot, O.; Blanco, M. -J.; Colesse, G.; Gourmelin, Y.

Blood Coagulation & Fibrinolysis: June 2001 - Volume 12 - Issue 4 - p 229-236
Original Article

This study reports on the frozen stability of all commonly measured coagulation proteins in normal citrated plasma: activated partial thromboplastin time, prothrombin time (%), thrombin time and fibrinogen (Clauss); clotting assays for factors II, V, VII, VIII, IX, X, XI and XII; functional assays for protein C (clotting), protein S (clotting), antithrombin (chromogenic) and plasminogen (chromogenic); and immunological assays for von Willebrand factor and D-dimer. All these factors listed are stable for up to 3 months if frozen at −24°C or lower. At −74°C, all these factors (allowing for 10% variation) were stable for at least 18 months, most were stable for 24 months. The number of proteins showing > 5% variation over baseline after 6 months storage indicates that some decay does occur even at −74°C. There was no clear advantage in snap freezing at −74°C and then storing at −24°C over both freezing and storing at −24°C; therefore, the freezing process itself is not responsible for the loss of stability. The best stability, especially at −24°C, was obtained when small samples (1 ml) were stored in screw-cap tubes with a minimum dead space. The decrease in stability of the coagulation proteins directly correlates with the effect of temperature and time.

B. Woodhams is with Serbio (Diagnostica Stago), Gennevilliers, France; and O. Girardot, M.-J. Blanco, G. Colesse and Y. Gourmelin are with Diagnostica Stago, Franconville, France.

(Received 25 September 2000;

revised 16 January 2001; accepted 17 January 2001)

Address correspondence to Yves Gourmelin, Diagnostica Stago, 2 rue Pierre Fossati, 95131 Franconville, France. Tel: (+33) 1 30 72 36 46; fax: (+33) 1 34 13 59 39; e-mail:

© 2001 Lippincott Williams & Wilkins, Inc.