Mutation report: PDF OnlySimple and rapid combined genetic diagnosis of mutation (1691 G→A) of the factor V gene and (20210 G→A) of the prothrombin geneDupérat, V. Guyonnet; Fauchon, M.; Nurden, A. T.; Vergnes, C.Author Information V. Guyonnet Dupérat and A. T. Nurden are with the CNRS UMR 5533, Hôpital Cardiologique, Avenue Magellan, 33604 Pessac, and M. Fauchon and C. Vergres are with the Laboratoire d'Hémobiologie, Hôpital Cardiologique, Avenue Magellan, 33604 Pessac, France. Blood Coagulation & Fibrinolysis: September 1998 - Volume 9 - Issue 6 - p 549-552 Buy Abstract We have developed a rapid method which allows us simultaneously to determine two genetic variations that are associated with an increased risk of venous thrombosis: the 20210 G→A mutation present in the 3′-UT region of the prothrombin gene and the 1691 G→A mutation giving rise to factor V Leiden. Our strategy involves the coamplification of exon 10 of the factor V gene and of the region 3′ from the prothrombin gene using modified oligonucleotides, permitting the introduction of a single Hind III cleavage site in fragments bearing one of the mutations. As a result of its time- and cost-saving features, this combined method should be considered for screening large numbers of patients. © 1998 Lippincott Williams & Wilkins, Inc.