Review ArticlesAntinociceptive effects of minor cannabinoids, terpenes and flavonoids in CannabisRodriguez, Carl Erwin B.,*; Ouyang, Lisa,*; Kandasamy, Ram Author Information Department of Psychology, California State University, East Bay, Hayward, California, USA *Carl Erwin B. Rodriguez and Lisa Ouyang contributed equally to the writing of this article. Received 3 August 2020 Accepted as revised 11 January 2021 Correspondence to Ram Kandasamy, PhD, Department of Psychology, California State University, East Bay, 25800 Carlos Bee Boulevard, Science S229, Hayward, CA 94542, USA, E-mail: [email protected] Behavioural Pharmacology: April 2022 - Volume 33 - Issue 2&3 - p 130-157 doi: 10.1097/FBP.0000000000000627 Buy Metrics Abstract Cannabis has been used for centuries for its medicinal properties. Given the dangerous and unpleasant side effects of existing analgesics, the chemical constituents of Cannabis have garnered significant interest for their antinociceptive, anti-inflammatory and neuroprotective effects. To date, Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) remain the two most widely studied constituents of Cannabis in animals. These studies have led to formulations of THC and CBD for human use; however, chronic pain patients also use different strains of Cannabis (sativa, indica and ruderalis) to alleviate their pain. These strains contain major cannabinoids, such as THC and CBD, but they also contain a wide variety of cannabinoid and noncannabinoid constituents. Although the analgesic effects of Cannabis are attributed to major cannabinoids, evidence indicates other constituents such as minor cannabinoids, terpenes and flavonoids also produce antinociception against animal models of acute, inflammatory, neuropathic, muscle and orofacial pain. In some cases, these constituents produce antinociception that is equivalent or greater compared to that produced by traditional analgesics. Thus, a better understanding of the extent to which these constituents produce antinociception alone in animals is necessary. The purposes of this review are to (1) introduce the different minor cannabinoids, terpenes, and flavonoids found in Cannabis and (2) discuss evidence of their antinociceptive properties in animals. Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.