Research ReportsExploratory analyses of postanesthetic effects of desflurane using behavioral test battery of miceNiikura, Ryoa,,*; Miyazaki, Tomoyukia,,b,,*; Yonezaki, Kumikoa,,*; Uchimoto, Kazuhiroa; Takase, Kenkichic; Goto, TakahisaaAuthor Information Departments of aAnesthesiology bPhysiology, Yokohama City University Graduate School of Medicine, Yokohama cLaboratory of Psychology, Jichi Medical University, Shimotsuke, Japan *Dr. Ryo Niikura, Dr. Tomoyuki Miyazaki, and Dr. Kumiko Yonezaki contributed equally to the writing of this article. Received 30 October 2019 Accepted as revised 14 April 2020 Supplemental Digital Content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal’s website, www.behaviouralpharm.com. Correspondence to Kenkichi Takase, PhD, Jichi Medical University, Yakushiji 3311-1, Shimotsuke-shi, Tochigi-ken 329-0498, Japan, E-mail: [email protected] Behavioural Pharmacology: October 2020 - Volume 31 - Issue 7 - p 597-609 doi: 10.1097/FBP.0000000000000567 Buy SDC Metrics Abstract Halogenated ethers, such as desflurane, sevoflurane, and isoflurane, are known to exert an array of effects besides sedation. However, the postanesthetic effects of desflurane remain undiscovered as no study has explored these effects systematically. Phenotypic screening using behavioral test batteries is a powerful method to identify such effects. In the present study, we behaviorally phenotyped desflurane-treated mice to investigate postanesthetic effects. We applied comprehensive behavioral test batteries measuring sensorimotor functions, anxiety, depression, sociability, attention, and learning abilities, starting 7 days after anesthesia performed with 8.0% desflurane for 6 h. Although our previous study revealed postanesthetic effects of isoflurane in adult mice, in the current study, desflurane-treated mice exhibited no such effects in any behavioral test. To further examine whether desflurane affect behavior in more early time point, we built up a new additional test battery, which carried out 1 day or 3 days after exposure to desflurane. Mice treated with desflurane 1 day before testing showed more slips than other two groups in the first trial, suggesting mild acute side effects of desflurane on motor coordination. These results suggest the safety of desflurane in clinical settings and imply that postanesthetic effects are unique to each halogenated ether. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.