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Efficacy of a Surfactant-Based Wound Dressing in the Prevention of Biofilms

Salisbury, Anne-Marie, PhD; Percival, Steven L., PhD

doi: 10.1097/01.ASW.0000544612.28804.34
FEATURES: ORIGINAL INVESTIGATION

OBJECTIVE: To assess the biofilm prevention action of two wound dressings, a concentrated surfactant gel preserved with antimicrobials and a concentrated surfactant gel with 1% silver sulfadiazine.

METHODS: The microorganisms Staphylococcus aureus, methicillin-resistant S aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Enterococcus faecalis were used. Several biofilm models were used whereby the surfaces of each model were coated with either the concentrated surfactant gel preserved with antimicrobials or the concentrated surfactant gel with SSD before biofilm growth.

MAIN RESULTS: Results showed the concentrated surfactant gel with SSD prevented biofilm growth in the modified minimum biofilm eradication concentration and Centers for Disease Control and Prevention biofilm models. The concentrated surfactant gel preserved with antimicrobials prevented microbial penetration for up to 48 hours, whereas the concentrated surfactant with SSD prevented microbial penetration for at least 72 hours. Using confocal laser scanning microscopy, researchers showed that a surface coated with the concentrated surfactant gel preserved with antimicrobials enhanced microbial sequestration of planktonic microorganisms.

CONCLUSIONS: These results demonstrated that a concentrated surfactant gel preserved with antimicrobials can sequester and cause the immobilization of planktonic bacteria. Further, the concentrated surfactant gel with SSD can effectively kill planktonic and sessile microorganisms.

At 5D Health Protection Group Ltd, Liverpool, United Kingdom, Anne-Marie Salisbury, PhD, is the Laboratory Manager and Senior Microbiologist; and Steven L. Percival, PhD, is Chief Executive Officer. Acknowledgments: The authors thank the Liverpool Centre for Cell Imaging at the University of Liverpool for their support. 5D Health Protection Group Ltd received a grant and supplies (PluroGel Burn and Wound Dressing and PluroGel with 1% silver sulfadiazine) from Medline Industries Inc to carry out the enclosed research. The authors have disclosed no other financial relationships related to this article. Submitted December 8, 2017; accepted in revised form March 15, 2018.

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