FEATURES: ORIGINAL INVESTIGATIONThe Effect of Vacuum-Assisted Closure Therapy on Methicillin-Resistant Staphylococcus aureus Wound BiofilmsĆirković, Ivana MD, PhD; Jocić, Dario MD, MS; Božić, Dragana D. MD, PhD; Djukić, Slobodanka MD, PhD; Konstantinović, Neda MD; Radak, Djordje MD, PhDAuthor Information In Belgrade, Serbia, Ivana Ćirković, MD, PhD, is Professor of Microbiology, Institute of Microbiology, University of Belgrade; Dario Jocić, MD, MS, is a vascular surgeon, Institute of Cardiovascular Diseases; Dragana D. Božić, MD, PhD, is Professor of Microbiology, Department of Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade; Slobodanka Djukić, MD, PhD, is Professor of Microbiology, Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade; Neda Konstantinović, MD, is a medical doctor, Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade; and Djordje Radak, MD, PhD, is Chief of Vascular Surgery, Institute of Cardiovascular Diseases, and Professor of Surgery, Faculty of Medicine, University of Belgrade. Acknowledgments: This research was supported by the Ministry of Education, Science and Technological Development, Republic of Serbia (project no. ON175039). The authors have disclosed no other financial relationships related to this article. Submitted July 26, 2017; accepted in revised form January 2, 2018. Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal’s Web site. Advances in Skin & Wound Care: August 2018 - Volume 31 - Issue 8 - p 361-364 doi: 10.1097/01.ASW.0000540070.07040.70 Buy SDC Metrics Abstract Biofilm-associated wound infections are a major global health issue, and methicillin-resistant Staphylococcus aureus (MRSA) is among the greatest therapeutic challenges. Vacuum-assisted closure (VAC) therapy is now being revisited as an alternative treatment for both acute and chronic wounds. However, data supporting the concept of its antibiofilm effect remain limited. Using quantitative biofilm-forming assay and a range of genotypic methods (spa, SCCmec, and agr typing), study authors showed that VAC therapy can significantly prevent biofilm formation (P < .01) of a range of MRSA wound isolates differing widely in their biofilm-forming abilities and genetic background. The best effect was presented on CC5-MRSA-SCCmecI-agrII, a dominant MRSA clone among wound isolates worldwide. An assessment of effects of different protocols on dressing changes (1 or 2 times per week) demonstrated significantly greater antibiofilm activity (P < .05) of 3-day dressing changes. These findings support the use of VAC therapy as a topical antibiofilm treatment for the effective management of wound healing. Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.