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Wound Healing Studies Using Punica granatum Peel: An Animal Experimental Study

Zekavat, Omidreza MD; Amanat, Aida MD; Karami, Mohammadyasin MD; Paydar, Shahram MD; Gramizadeh, Bita PhD; Zareian-Jahromi, Maryam MD

Advances in Skin & Wound Care: May 2016 - Volume 29 - Issue 5 - p 217–225
doi: 10.1097/01.ASW.0000481116.16998.55
FEATURES: ORIGINAL INVESTIGATION

OBJECTIVE: The goal of the present study was to evaluate the effects of hydroalcoholic extract-based carboxy methyl cellulose (CMC) gel of Punica granatum peel (PCMC) and CMC on healing of full-thickness skin wounds.

MATERIALS AND METHODS: Forty-two rats were studied. Each rat had 3 wounds that were treated topically with PCMC as the case, CMC as the positive control, and sterile saline as the negative control. All 3 wounds of each rat were photographed during the wound healing period at days 0 (onset of wound surgery), 3, 6, 9, and 12.The wound area was calculated using Adobe Photoshop CS (version 5) software (Adobe Systems Inc, San Jose, California). Electrocardiogram paper was used for reference scale.

RESULTS: The results of this study show that macroscopic and microscopic wound healing took a significantly longer time in wounds treated with normal saline than those treated with PCMC (grossly) and CMC gel (grossly and significantly).

CONCLUSIONS: The authors’ findings show that anti-inflammatory, antihemorrhagic, and antinecrotic effects of CMC lead to early healing of skin wounds.

Omidreza Zekavat, MD, is an Assistant Professor in the Department of Pediatrics Hematology and Oncology; Aida Amanat, MD, is a Medical Student; and Mohammadyasin Karami, MD, is a Medical Student; all at the Jahrom University of Medical Sciences in Jahrom, Fars, Iran. Shahram Paydar, MD, is an Assistant Professor in the Trauma Research Center, and Bita Gramizadeh, PhD, is a Professor in the Department of Pathology at Shiraz University of Medical Sciences in Shiraz, Fars, Iran. Maryam Zareian-Jahromi, MD, is a student of medicine at the Jahrom University of Medical Sciences in Jahrom, Fars, Iran.

Acknowledgments: The authors thank Jahrom University of Medical Science for its financial support; Seyyed Maryam Zekavat at the Department of Biological Engineering, Massachusetts Institute of Technology for editorial assistance on the manuscript, and Omid Koohi at the Animal Laboratory of Shiraz University of Medical Sciences for maintaining the animals during the study. The authors have disclosed that they have no financial relationships related to this article.

Submitted April 29, 2014; accepted in revised form November 21, 2014.

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