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Abstracts: ASAIO Bioengineering/tissue Engineering Abstracts

TISSUE ENGINEERING OF HEART VALVES: A NEW DYNAMIC CELL SEEDING DEVICE FOR TISSUE ENGINEERING OF HEART VALVES

Lueders, Cora1; Sodian, Ralf1; Hetzer, Roland1

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Objective:

A crucial factor in the tissue engineering of heart valves, is an effective cell seeding with uniform cell distribution on biodegradable scaffolds to eventually form functional tissue constructs in vitro. In our laboratory we developed a new cell seeding device for optimal cell distribution for tissue engineered heart valve constructs.

Methods and Results:

In the present study we developed a new cell seeding device which is made of acrylic glass and completely transparent. The polymeric heart valve scaffold is fixed in a small volume, cylindrical cell seeding chamber, and is surrounded by optimal cell suspension. The cell seeding chamber is placed in an acrylic glass bowl so that it can rotated in all directions to provide optimal cell distribution to all areas of the heart valve construct. We thus developed a highly isolated cell seeding device which is driven by an independently developed rotating machine consisting of two independent motors. The whole system provides a high level of sterility and fits into a humidified incubator.

Conclusion:

Our newly developed cell seeding device provides sterile conditions and optimal cell distribution for the controlled fabrication of autologous tissue engineered heart valve constructs.

Copyright © 2005 by the American Society for Artificial Internal Organs