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Abstracts: ASAIO Bioengineering/tissue Engineering Abstracts

STRUCTURE INFLUENCES CELLULAR COMPATIBILITY IN A MICROPOROUS POLYURETHANE SCAFFOLD

Yaguchi, Toshiyuki1; Funakubo, Akio1; Okoshi, Takafumi2; Noishiki, Yasuharu3; Fukui, Yasuhiro1

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Endothelial cells play a key role in preventing blood coagulation. Almost all adherent cells including endothelial cells are unable to multiply in a liquid without a scaffold even in the presence of sufficient factors such as growth factors and cytokines. Cells have the unique characteristic of scaffold-dependent attachment when they contact a structure. In fact, fibroblasts can attach to and divide on microfiber and we have successfully implanted a microporous vascular graft (ϕ1.5mm) into the rat abdominal aorta. We therefore postulated that a fibrous microporous structure would be a suitable scaffold for cell adherence and growth. Our aim is to identify a highly cytotropic scaffold that can utilize cytotaxis. An optimal scaffold would have a broad range of applications such as artificial anti-thrombotic organs and small diameter vascular grafts. Here, we examined the influence of production conditions on scaffold structures and that of the structure on the growth of fibroblast. Our results showed that the ratio of the fiber area can be altered ranging from 20 to 50% by fabrication conditions such as lengthening the mandrill-spray distance and by injecting flow rate of the polyurethane solution. We cultured fibroblast on a microfiber scaffold in vitro and confirmed that the numbers of various control cell types that thrived on the support were essentially identical. Further studies of the relationship between 3-dimensional scaffold structures and cellular activation are in progress.

Copyright © 2005 by the American Society for Artificial Internal Organs