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Abstracts: ASAIO Bioengineering/tissue Engineering Abstracts

REAL TIME IMAGING OF HEMOLYSIS IN IN-VITRO WITH CHEMICAL LUMINESCENCE

Mochizuki, Shuichi1; Takiura, Koki2; Abe, Yusuke1; Imachi, Kou2

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Present methods that evaluate the hemolysis in blood pumps employ circulation of blood and measurement of free hemoglobin. It cannot evaluate the occurrence and location of hemolysis. In this study, chemical luminescence induced by hemolysis was detected with CCD camera and hemolysis was observed in real time imaging. The Fe complex as hemoglobin catalyzed the chemical luminescence of Luminol and O2. Hemoglobin from the hemolytic erythrocyte renders this reaction, which generates faint light. The hemolysis can be detected by faint light. A small amount of Luminol and H2O2 were added to goat blood. Water was added to this mixture to generate hemolysis while the faint light was observed with high sensitivity CCD camera. This CCD camera can take images as 30 frames / sec. Hemoglobin solutions (0μg/dl to 100μg/dl in goat plasma) were added to a mixture of Luminol and H2O2, and video images were recorded at each concentration of hemoglobin. A centrifugal blood pump (HPM-05, Nikkiso) filled with diluted goat blood, Luminol and H2O2, while the images were taken when pump drive. In the goat blood with Luminol and H2O2, there is no observation of faint light. After water added into the mixture, faint light can be detected with CCD camera and it can draw the flow pattern of injected water. Hemolysis occurrence can be detected by CCD camera. The higher hemoglobin concentration, the higher intensity of image pixels can be observed. CCD imaging of faint light comes from Pump head is presently measured to determine where hemolysis occurs in real time. Analysis of intensity can be also estimated the hemolysis quantity.

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