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Abstracts: ASAIO Bioengineering/tissue Engineering Abstracts


Narine, Kishan K1; Claeys, Erik1; Van Langenhove, Lieva1; Van Nooten, Guido1

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Clinical use of seeded tissue engineered porcine valves would require them to be available on short notice. Currently available technology however, is time consuming, requiring the procurement of a suitably sized valve and subsequent decellularisation and quality control before repopulation. We examined the biomechanical properties of fresh porcine valves and of matrices before and after cryopreservation. Matrices prepared according to a modified enzymatic-detergent decellularisation protocol. Tissue mechanical properties were determined by multi-axial tensile measurements and their biochemical status by collagen and uronic acid determination (proteoglycan). Histological and ultrastructural properties were evaluated by light and electron microscopy. Although the average maximum force at failure of matrices before and after cryopreservation was significantly higher than in fresh tissue the Breakage Force was not significantly different in fresh tissue and matrices. There was no significant difference between the stiffness of matrices and fresh tissue. Matrices themselves did differ in the transition phase which was significantly longer in matrices after cryopreservation. Neither the collagen nor uronic acid content were significantly different before and after cryopreservation of aortic leaflets matrices. Histological and ultrastructural sections confirmed the structural integrity of matrices before and after cryopreservation. Cryopreservation offers a potential means of ensuring readily available scaffolds of various sizes and reliable strength for use in tissue valve engineering.

Copyright © 2005 by the American Society for Artificial Internal Organs