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Abstracts: ASAIO Bioengineering/tissue Engineering Abstracts


Mareels, Guy1; Eloot, Sunny1; Poyck, Paul2; Chamuleau, Rob A2; Verdonck, Pascal R1

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Bioartificial livers (BAL), like the AMC BAL (Academic Medical Center, Amsterdam, The Netherlands) have been used to bridge patients with acute liver failure to liver transplantation or regeneration. Improvement of the liver specific function of the charged hepatocytes to increase the clinical applicability of these BAL systems might be achieved by analyzing and optimizing the plasma perfusion and oxygen (O2) supply to the hepatocytes. A complete 3D computer model of the BAL and subsequent computational fluid dynamics simulations (Fluent 6) allow to study the detailed flow field and local O2 transport. The AMC BAL comprises a spirally wound non-woven polyester matrix for high-density hepatocyte culture and uses gas capillaries for O2 supply. The matrix is modelled as a homogeneous porous medium and is implemented either as a subpart of the geometry (full model) or using a user-controlled variable which marks its location in the finite volume mesh (abstracted model). Hepatocyte O2 consumption is modelled using non-linear Michaelis-Menten kinetics. Two fully parametric 3D models of the AMC BAL were developed to allow future parametric design studies. The abstracted model is flexible to use, but results in jagged matrix edges. The full model has a well-defined geometry but is time consuming to build. Simulations show preferential flow paths and a not completely homogeneous O2 distribution. The abstracted and full model can be compared for solution accuracy. The developed models are useful to further optimize the transport processes inside the AMC BAL and other bioreactors.

Copyright © 2005 by the American Society for Artificial Internal Organs