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Abstracts: ASAIO Bioengineering/tissue Engineering Abstracts

LIPOSOME-ENCAPSULATED HEMOGLOBIN WITH A HIGH OXYGEN AFFINITY ACCELERATES GASTRIC WOUND HEALING IN THE RAT

Okamoto, Yuichi1; Kawaguchi, Akira T1; Kise, Hiroyuki1; Izumikubo, June1; Ogoshi, Kyouji1; Imaizumi, Toshihide1; Makuuchi, Hiroyasu1

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Background.

There have been few direct measures to improve surgical wound healing. We hypothesize that liposome-encapsulated hemoglobin with a high O2 affinity (hLEH) efficiently carry O2 to the hypoxic wound and improve its healing.

Methods.

hLEH (n=27), empty liposome (EL, n=20), and homologous transfusion (TX, n=17) were administered prior to 1 cm gastrotomy and reanastomosis with 4 interrupted stitches in SD rats, which were killed 2 and 4 days later to measure bursting pressure of the anastomosis. Excised wound underwent pathologic studies.

Results.

While bursting pressure was significantly higher in 2 days in hLEH-treated rats than in animals receiving transfusion or empty liposome, there was no difference in 4 days (Figure). Histologic examination revealed less inflammation and more granulation in hLEH-treated rats with a significantly more macrophages at around the anastomosis in hLEH-treated animals.

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Figure

Conclusion.

The current results support the hypothesis that hLEH before gastrotomy facilitate wound healing not because of hemoglobin or liposome capsule but because of hLEH per se. Histologic studies also support better wound healing by hLEH, which may be phagocytosed by macrophages.

Copyright © 2005 by the American Society for Artificial Internal Organs