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Abstracts: ASAIO Bioengineering/tissue Engineering Abstracts

EXPERIMENTAL STUDY ON SCAFFOLD BASED TISSUE REGENERATION OF THE ESOPHAGUS USING CHITIN

Hori, Yoshio1; Sekine, Kazumitsu2; Shiraishi, Yasuyuki2; Inagaki, Akiko1; Yambe, Tomoyuki2

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Purpose of study:

We have tried tissue reconstruction of the esophagus. Recently, various applications were reported using chitin as biomaterials such as artificial skin substitutes and so on. We hypnotized that chitin could be applied as a scaffold for guiding esophagus tissue regeneration. The aim of this study is to evaluate the feasibility of chitin scaffold based esophagus regeneration.

Methods used:

To evaluate the feasibility of chitin sheet guided esophagus regeneration, we carried out experiment using goat model. The experiment was chitin scaffold replacement in cervical esophagus for the portion of 6cm long as scaffold based tissue engineering expecting host cells migration into the scaffold. The animal was fasted and kept with intravenous hyperalimentation. We evaluated the regenerated esophagus at 4 weeks after surgery macroscopically and histologically.

Summarize results:

Tubular tissue regeneration was observed at 4 weeks after implantation. Epithelium layer covered the luminal surface of the regenerated esophagus partially. However, the muscle layer regeneration was not observed as previously reported using other scaffold such as collagen. The results showed the possibility of the chitin scaffold based esophagus regeneration.

Copyright © 2005 by the American Society for Artificial Internal Organs