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ASAIO CARDIAC ABSTRACT

INVESTIGATION OF MICRO-SCALE BLOOD FLOW BEHAVIOR AND SHEAR-INDUCED BLOOD DAMAGE BY CELL TRACKING VELOCIM-ETRY

Zhao, R1; Antaki, J F2; Wu, Z J3; Kameneva, M V1; Bachman, T N1

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Trauma to formed elements of blood within prosthetic cardiovascular devices often occurs within small passages or journals of the flow path. These regions may be associated with 'hot-spots' in which shear stress is excessive and cells may be irreversibly strained. The successful design of these devices relies on efficiently minimizing supra-physiologic shear fields through computational modeling. However the fundamental blood mechanics under these conditions are not yet fully characterized. This study was therefore conducted to elucidate the microscopic mechanics of cellular deformation that underlie shear-induced hemolysis. A micro fluid system was developed to emulate flow environments at 'hot spots' and provide optical access for microscopic visualization. The flow of both bovine and rodent red blood cells (RBCs) within micro channels was illuminated by a pair of xenon stroboscopes resulting in a rapid succession of images – recorded by double-exposure digital CCD camera. The dynamic alterations of the RBC dimensions and the distribution of the cell free layer were analyzed under various conditions of hematocrit, flow rate, and channel geometry. The velocity profile of RBCs was also determined using a cell tracking velocimetry algorithm

Copyright © 2004 by the American Society for Artificial Internal Organs