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ASAIO CARDIAC ABSTRACT

BLOOD VESSEL REMODELING BY GAS-PLASMA TREATED SCAFFOLDS IN AGED NUDE MICE

Polan, I L1; Munoz, O1; Agrawal, C M2; Bailey, S R1

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Purpose: Increased blood vessel remodeling occurs from endothelial-containing bioresorbable gas-plasma treated polylactide acid (D, L-PLA) scaffolds when implanted into 2-month nude mice (N = 114). Our hypothesis is that endothelial-containing gas-plasma treated scaffolds similarly implanted within 14-month old nude mice will exhibit vascular remodeling induced from Vascular Endothelial Growth Factor (VEGF) and basic Fibroblast Growth Factor (bFGF). Methods: 14-month aged immunological-deficient nude mice were implanted with non-treated control (n = 7], gas-plasma treated bioresorbable scaffolds (n = 8) containing human aortic endothelial cells or were procedural shams (n = 2). Scaffolds and peritoneal tissues were evaluated by in situ quantification of blood vessels (scale 0-4) at 72 days and scores statistically compared. In addition, specimens were frozen for protein extractions and Western Blots for VECF and bFCF. Results: Treated scaffolds induced blood vessel densities near the scaffolds that averaged 1.83±0.R; whereas, control scaffolds induced 1.47±0.9. Consequently, ANOVA showed no statistical difference (p=0.55). Shams scored an average of 0±0.0. Both treated (p=0.01 7) and control (p = 0.031) vessel densities were significantly different from the shams. Blood vessel densities above and near the scaffolds were less than in the earlier study using 2-month mice. Western Blots are still ongoing but will be presented. Conclusion: 17-month aged nude mice exhibit reduced blood vessel remodeling when implanted with endothelial-containing gas-plasma and non-treated D, L-PLA scaffolds.

Copyright © 2004 by the American Society for Artificial Internal Organs