This article presents a primed left ventricle heart perfusion method to generate physiologic aortic pressure (AoP) and perform functional assessment. Isolated hearts of male Yorkshire pigs were used to study the hemodynamic behaviors of AoPs generated in the primed left ventricle heart perfusion (n = 6) and conventional (zero-loaded left ventricle) Langendorff perfusion (n = 6). The measurement results show that left ventricular pressure generated in the primed left ventricle heart perfusion is a determinant of physiologic AoP (i.e. systolic and diastolic pressures within physiologic range). The aortic pulse pressure (systolic pressure = 124.5 ± 1.7 mm Hg, diastolic pressure = 87.8 ± 0.9 mm Hg, aortic pulse pressure = 36.7 ± 2.6 mm Hg) from the primed left ventricle heart perfusion represents close match with the in vivo physiologic data. The volume in the left ventricle remains constant throughout the primed left ventricle heart perfusion, which allows us to perform isovolumetric left ventricular pressure measurement in ex vivo heart perfusion (EVHP). Left ventricular contractility measurements (maximum and minimum rates of left ventricular pressure change) were derived for cardiac assessment. In summary, the proposed primed left ventricle heart perfusion method is able to create physiologic AoP and enables left ventricular functional assessment in EVHP in porcine hearts.
From the *Department of Mechanical & Industrial Engineering, University of Toronto, Toronto, Ontario Canada
†Department of Cardiovascular Surgery, University Health Network, Canada
‡School of Astronautics, Harbin Institute of Technology, Harbin, China
§School of Mechatronic Engineering and Automation, Shanghai University, China
¶Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada
‖Schaefer School of Engineering and Science, Stevens Institute of Technology.
Submitted for consideration September 2018; accepted for publication in revised form December 2018.
Disclosure: The authors declare no conflicts of interest.
Correspondence: Yu Sun, 5 King’s College Rd, Toronto, Ontario, Canada. Email: email@example.com; and Mitesh Badiwala, 4N-472, 200 Elizabeth Street, Toronto, Ontario, Canada. Email: firstname.lastname@example.org.