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Enhanced Osteogenic Differentiation of Mesenchymal Stem Cells on Electrospun Polyethersulfone/Poly(Vinyl) Alcohol/Platelet Rich Plasma Nanofibrous Scaffolds

Kashef-Saberi, Mahshid Sadat*; Hayati Roodbari, Nasim*; Parivar, Kazem*; Vakilian, Saeid; Hanaee-Ahvaz, Hana

doi: 10.1097/MAT.0000000000000781
Tissue Engineering/ Biomaterials

Over the last few decades, great advancements have been achieved in the field of bone tissue engineering (BTE). Containing a great number of growth factors needed in the process of osteogenesis, platelet rich plasma (PRP) has gained a great deal of attention. However, due to the contradictory results achieved in different studies, its effectiveness remains a mystery. Therefore, in this study, we investigated in vitro performance of co-electrospun PRP/poly ether sulfone/poly(vinyl) alcohol (PRP/PES/PVA) composite scaffolds for the osteogenic differentiation of human adipose-derived mesenchymal stem cells. The activated PRP was mixed with PVA solution to be used alongside PES solution for the electrospinning process. Fourier transform infrared spectroscopy, scanning electron microscopy and tensile tests were performed to evaluate the scaffolds. After confirmation of sustained release of protein, osteogenic potential of the co-electrospun PRP/polymer scaffolds was evaluated by measuring relative gene expression, calcium content, and alkaline phosphatase (ALP) activity. Alizarin red and Hematoxylin and Eosin staining were performed as well. The results of ALP activity and calcium content demonstrated the effectiveness of PRP when combined with PRP-incorporated scaffold in comparison with the other tested groups. In addition, the results of tensile mechanical testing indicated that addition of PRP improves the mechanical properties. Taking these results into account, it appears PES/PVA/PRP scaffold treated with PRP 5% enhances osteogenic differentiation most. In conclusion, incorporation of PRP into electrospun PES/PVA scaffold in this study had a positive influence on osteogenic differentiation of AdMSCs, and thus it may have great potential for BTE applications.

From the *Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Department of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, Iran.

Submitted for consideration September 2017; accepted for publication in revised form February 2018.

Disclosure: The authors declare no conflict of interest.

This work was supported by Stem Cell Technology Research center.

Supplemental digital content is available for this article. Direct URL citations appear in the printed text, and links to the digital files are provided in the HTML and PDF versions of this article on the journal’s Web site (www.asaiojournal.com).

Correspondence: Hana Hanaee-Ahvaz, Stem Cell Technology Research Center, Tehran, Iran. Email: Hanaee@STRC.ac.ir.

Copyright © 2018 by the American Society for Artificial Internal Organs