Research Articlesp53-Family Proteins in Odontogenic Cysts: An Immunohistochemical StudyMascitti, Marco DDS, MD*; Togni, Lucrezia DDS*; Balercia, Andrea DDS†; Balercia, Paolo MD†; Rubini, Corrado MD‡; Santarelli, Andrea DDS*,§Author Information *Department of Clinical Specialistic and Dental Sciences †Department of Oral and Head-Neck Surgery, Umberto I General Hospital ‡Department of Biomedical Sciences and Public Health, Marche Polytechnic University §National Institute of Health and Science of Aging, INRCA, Ancona, Italy The authors declare no conflict of interest. Reprints: Marco Mascitti, DDS, MD, Via Tronto 10, Ancona 60126, Italy (e-mail: [email protected]). Applied Immunohistochemistry & Molecular Morphology: May/June 2020 - Volume 28 - Issue 5 - p 369-375 doi: 10.1097/PAI.0000000000000727 Buy SDC Metrics Abstract The present study investigated the immunohistochemical expression of p53, p63, and p73 in different types of odontogenic cysts (OC), a group of common intraosseous jaw lesions, to provide a better understanding of p53-family functions in odontogenic lesions. We carried out immunohistochemical analysis to evaluate the expression of p53, p63, and p73 in 60 samples of OC, including dentigerous cysts, radicular cysts, orthokeratinized OC, and odontogenic keratocysts (OKC). The epithelial expression of p53-family members was evaluated both in the basal-parabasal and in the superficial layers, measuring the percentage of positive cells and the value of expression intensity. The expression of p53-family members showed a significant difference between the “OKC” and “non-OKC” groups. In particular, p53 positivity in the basal-parabasal layers, as well as p63 positivity in the superficial layers, were more common in OKC (P<0.0001; P=0.0237). p73 expression in the superficial layers was significantly more expressed in the “non-OKC” group (P<0.0001). No significant differences of staining intensity scores were reported between the groups. The Spearman test showed a positive correlation between p53 and p73 expression at the basal-parabasal level in all cysts (r=0.6626; P<0.0001). These results showed a significantly different expression of p53-family members in OC groups, in particular between the “OKC” and “non-OKC” groups, suggesting the existence of a p53-family pathway in the epithelial lining of OC. Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.