Technical ArticleRecommendations for Tissue Microarray Construction and Quality AssuranceGlinsmann-Gibson, Betty MS*; Wisner, Lee AA*; Stanton, Melissa MD†; Larsen, Brandon MD, PhD†; Rimsza, Lisa MD†; Maguire, Alanna PhD*Author Information Departments of *Research †Laboratory Medicine and Pathology, Mayo Clinic Arizona, Scottsdale, AZ Source of Funding: NIH/NCI UM1 CA181255-2. The authors declare no conflict of interest. Reprints: Betty Glinsmann-Gibson, MS, CRB 01-250A, Mayo Clinic Arizona, 13400 East Shea Blvd, Scottsdale, AZ 85259 (e-mail: email@example.com). Applied Immunohistochemistry & Molecular Morphology: April 2020 - Volume 28 - Issue 4 - p 325-330 doi: 10.1097/PAI.0000000000000739 Buy Metrics Abstract Tissue microarrays (TMAs) are important tools to conserve precious tissue resources from increasingly smaller biopsies and to control experimental costs and variation across sample sets. The quality assurance assessment of TMA materials created at centralized biobanks has not been standardized. Herein, we outline 2 processes for the construction of TMAs (“recipient block” and “tape” methods) and the associated preconstruction quality control measures (pathology review, protein and RNA assessment, map creation, and storage conditions) developed by the AIDS Cancer Specimen Resource (ACSR) Network’s Science and Technology Core. These steps provide a suggested framework for quality assessment that allows end-users, receiving materials from tissue banks, confidence in their experimental results. Copyright © 2019 Wolters Kluwer Health, Inc. All rights reserved.