This review article summarized recent advances in the heat-induced antigen retrieval technique with numerous scientific fields in addition to immunohistochemistry. Particularly, proteomics including imaging mass spectrometry, extraction of proteins from formalin-fixed, paraffin-embedded (FFPE) tissues. Some novel approaches such as FFPE tissue–based renal immunopathology based on modified double heating protocols are also introduced in this review for further development. In general, the FFPE tissue housed in pathology worldwide is an invaluable treasure, and the simple method of heat-induced antigen retrieval is the gold key to open the door of this treasure.
*Department of Pathology, University of Southern California Keck School of Medicine, Los Angeles, CA
†Department of Pathology, New York University School of Medicine, New York, NY
‡Department of Pathology, Shantou University School of Medicine, Shantou, Guangdong Province, China
Supported by NIH grant 1 R42 CA122715, and 1R21 CA134359 from the Innovative Molecular Analysis Technologies (IMAT) Program.
The authors declare no conflict of interest.
Reprints: Shan-Rong Shi, MD, Department of Pathology, University of Southern California Keck School of Medicine, Los Angeles, CA 90033 (e-mail: firstname.lastname@example.org).
Received February 22, 2019
Accepted April 17, 2019