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Immunohistochemical Detection of γ/δ T Lymphocytes in Formalin-fixed Paraffin-embedded Tissues

Jungbluth, Achim A. MD, PhD*; Frosina, Denise BS*; Fayad, Miriam BS*; Pulitzer, Melissa P. MD*; Dogan, Ahmet MD*; Busam, Klaus J. MD*; Imai, Naoko MD, PhD; Gnjatic, Sacha PhD

Applied Immunohistochemistry & Molecular Morphology: September 2019 - Volume 27 - Issue 8 - p 581–583
doi: 10.1097/PAI.0000000000000650
Technical Article

T lymphocytes can be distinguished based on the composition of the T-cell receptor (TCR) chain in α/β T cells and γ/δ T cells. Correspondingly, α/β lymphomas can be distinguished from γ/δ lymphomas. The latter are rare neoplasms, which are usually confined to particular organs and tissues and carry a dismal prognosis. Until recently, monoclonal antibody (mAb) clone g3.20 to the TCR γ-chain was the reagent of choice for the immunohistochemical detection of γ/δ T cells and lymphomas in standard formalin-fixed paraffin-embedded tissues. However, due to technical problems, mAb g3.20 became recently unavailable. Our attempts to identify another commercially available clone to the TCR γ-chain were unsuccessful. However, we were able to identify a mAb (clone H-41, SC-100289; Santa Cruz, Dallas, TX) to the TCR δ-chain. H-41 works well in immunohistochemistry on paraffin-embedded tissue and comparison with previously stained cases, shows superior immunolabeling to mAb g3.20. H-41 to the TCR δ-chain appears to be a suitable reagent for the replacement of mAb g3.20.

*Department of Pathology, Memorial Sloan Kettering Cancer Center

Tisch Cancer Institute, Hematology/Oncology, Immunology, Icahn School of Medicine at Mount Sinai, New York, NY

The authors declare no conflict of interest.

Reprints: Achim A. Jungbluth, MD, PhD, Department of Pathology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065 (e-mail:

Received December 8, 2017

Accepted January 20, 2018

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