WIF-1 and Ihh Expression and Clinical Significance in Patients With Lung Squamous Cell Carcinoma and AdenocarcinomaZhang, Yue MD, PhD; Hu, Chunhong MD, PhDApplied Immunohistochemistry & Molecular Morphology: August 2018 - Volume 26 - Issue 7 - p 454–461 doi: 10.1097/PAI.0000000000000449 Research Articles Buy Abstract Author InformationAuthors Article MetricsMetrics This study investigated the expression of wingless-type inhibitory factor-1 (WIF-1) and Ihh protein in tumor tissues and their clinical significance in patients with lung squamous cell carcinoma and adenocarcinoma. The expression of WIF-1 and Ihh protein in 74 squamous cell carcinomas and 76 adenocarcinomas was measured by immunohistochemistry. The percentage of positive WIF-1 protein expression was significantly higher, while positive Ihh protein expression was significantly lower in patients with well-differentiated lung squamous cell carcinoma and adenocarcinoma, tumor node metastasis (TNM) stage I disease, and lymph node metastasis than that in patients with poorly differentiated tumor, TNM stage III disease, and lymph node metastasis (P<0.05, <0.01). Kaplan-Meier survival analysis showed that TNM stage and lymph node metastasis were significantly associated with the mean overall survival of patients with lung squamous cell carcinoma and adenocarcinoma (P<0.05 or <0.01). Patients with lung squamous cell carcinoma (P=0.037) and adenocarcinoma (P=0.001) with positive Ihh protein expression survived significantly shorter than patients with negative Ihh protein expression. In contrast, no significant difference in mean survival was observed in patients with lung squamous cell carcinoma and adenocarcinoma with positive and negative WIF-1 protein expression (P>0.05). Ihh is a marker for poor prognosis in patients with lung squamous cell carcinoma and adenocarcinoma. WIF-1 is not a predictive marker for lung cancer. Department of Oncology, Second Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China The authors declare no conflict of interest. Reprints: Chunhong Hu, MD, PhD, 139 Renmin Road, Changsha, Hunan 410011, P.R. China (e-mail: email@example.com). Received March 30, 2016 Accepted August 23, 2016 Copyright 2018 Wolters Kluwer Health, Inc. All rights reserved.