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Immunohistochemical Expression of CD105 and TGF-β1 in Oral Squamous Cell Carcinoma and Adjacent Apparently Normal Oral Mucosa and its Correlation With Clinicopathologic Features

Nair, Sindhu MDS*; Nayak, Ramakant MDS*; Bhat, Kishore MD; Kotrashetti, Vijayalakshmi S. MDS*; Babji, Deepa MDS*

Applied Immunohistochemistry & Molecular Morphology: January 2016 - Volume 24 - Issue 1 - p 35–41
doi: 10.1097/PAI.0000000000000152
Research Articles

Angiogenesis in oral squamous cell carcinomas (OSCC) is essential for its growth, invasion, and metastasis. This entails a shift in the balance between proangiogenic and antiangiogenic factors. CD105 and TGF-β1 are 2 such proangiogenic factors wherein CD105 exerts its angiogenic effect by binding to and modulating the TGF-β1 pathway. A total of 50 resected specimens of OSCC were considered. One tissue specimen was taken from tumor proper and another specimen from adjacent apparently normal mucosa (AANM). Both tissues were immunohistochemically stained using CD105 and TGF-β1 antibodies. The expression of each antibody was individually assessed and then compared. Pearson χ2 test was used for statistical comparison of expression. CD105 was significantly expressed in OSCC as compared with AANM and also correlated with increasing TNM stage. The mean microvessel density was higher in OSCC. TGF-β1 was significantly expressed in epithelium of OSCC as compared with AANM. On comparing expression of TGF-β1 and CD105, 79.54% of endothelial cells expressed positivity for both molecules. Both CD105 and TGF-β1 were increased in OSCC, although based on our results CD105 alone can be used as a prognostic marker. On the basis of immunohistochemical expression of CD105 and TGF-β1 in endothelial cells, our results demonstrate that CD105 acts as one of the receptors of TGF-β1 on endothelial cells and induces the angiogenic pathway in OSCC.

Departments of *Oral Pathology and Microbiology

Molecular Biology, Maratha Mandal’s NGH Institute of Dental Sciences, Belgaum, Karnataka, India

The authors declare no conflict of interest.

Reprints: Sindhu Nair, MDS, Department of Oral Pathology and Microbiology, Maratha Mandal’s NG Halgekar Institute of Dental Sciences, Belgaum 590010, Karnataka, India (e-mail:

Received July 23, 2014

Accepted September 6, 2014

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