Research ArticlesInvasion Phenotypes of Oral Squamous Cell CarcinomaEtemad-Moghadam, Shahroo DDS, MS; Alaeddini, Mojgan DDS, MSAuthor Information Dental Research Centre, Dental Research Institute, Dental Research Centre, Tehran University of Medical Sciences, Tehran, Iran S.EM and M.A. contributed equally. Supported by a grant from Tehran University of Medical Sciences: grant no. 132/297. The authors declare no conflict of interest. Reprints: Mojgan Alaeddini, DDS, MS, Dental Research Institute, Dental Research Centre, Ghods St, Enghelab Ave, P.O. Box: 14155-5583; Tehran 14174, Iran (e-mail: [email protected]). Received December 27, 2014 Accepted January 15, 2015 Applied Immunohistochemistry & Molecular Morphology: September 2015 - Volume 23 - Issue 8 - p e12-e16 doi: 10.1097/PAI.0000000000000184 Buy Metrics Abstract The invasion signature of tumor cells can strongly affect their metastatic behavior; therefore, we aimed to evaluate the different invasion phenotypes of oral squamous cell carcinoma (OSCC) and to determine the correlation of these markers with neoplastic mode of invasion. A total of 92 OSCCs were graded according to the Yamamoto criteria and subjected to immunohistochemistry using monoclonal antibodies against E-cadherin, N-cadherin, and podoplanin. Samples were grouped into epithelial-mesenchymal transition (EMT), collective-cell invasion (CCI), and combined intermediate phenotypes followed by statistical analysis. Significant differences were found among the 3 groups (P<0.001), between EMT and CCI (P=0.023) and between the combined phenotypes and both EMT and CCI, separately (P<0.001). Mode of invasion was significantly associated with E-cadherin (P=0.022), but not the other markers. There seems to be a preference for OSCCs to migrate by CCI; however, most our samples showed an intermediate phenotype between EMT and CCI, which confirm the existence of a spectrum of migration signatures that need to be further evaluated in these tumors. Copyright 2015 Wolters Kluwer Health, Inc. All rights reserved.