Research ArticlesThe Effect of Smoking on Endothelin-1 in Patients With Chronic PancreatitisŚliwińska-Mossoń, Mariola MD; Milnerowicz, Stanisław MD, PhD; Nabzdyk, Stanisław MD; Kokot, Izabela MSc; Nowak, Marcin MVD; Milnerowicz, Halina MD, PhDAuthor Information Departments of *Biomedical and Environmental Analysis †Gastrointestinal and General Surgery, Wrocław Medical University ‡Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Present address: Stanisław Nabzdyk, MD, Regional Specialist Hospital Wroclaw, Research and Development Centre, Clinic of Oncological Surgery, Poland. Supported by grant for young scientists No. 59/Pbmn awarded by the Wroclaw University of Medicine. The authors declare no conflict of interest. Reprints: Mariola Śliwińska-Mossoń, MD, Department of Biomedical and Environmental Analyses, Wroclaw University of Medicine, Borowska 211, Wroclaw 50-556, Poland (e-mail: [email protected]). Received July 30, 2013 Accepted February 16, 2014 Applied Immunohistochemistry & Molecular Morphology: April 2015 - Volume 23 - Issue 4 - p 288-296 doi: 10.1097/PAI.0000000000000077 Buy Metrics Abstract The aim of this study is to prove the influence of tobacco smoking on the endothelin-1 (ET-1) level in the plasma and on the immunohistochemical localization in the pancreatic tissues. The blood was collected from 50 healthy individuals and 63 patients with chronic pancreatitis (CP). The ET-1 and cotinine concentrations in the plasma were estimated by ELISA. Samples of tissues of the normal pancreas and CP were verified histopathologically, and then ET-1 was localized by immunohistochemical staining using the monoclonal anti-human ET-1 antibody. The intensity of immunohistochemical reaction was calculated with the semiquantitative Digital Imaging Methodology. The study demonstrated a significant concentration of ET-1 in smoking healthy individuals and in patients with CP when compared with the nonsmoking population (P=0.003 and 0.0005, respectively). A significantly stronger immunohistochemical ET-1 reaction was observed in the tissue of smoking patients with CP than in the normal pancreatic tissue and of nonsmoking CP patients (P=0.001, 0.008, and 0.03, respectively). The presented data evidence that tobacco smoking has a direct effect on the endothelium, leading to an increased level of ET-1. Copyright 2015 Wolters Kluwer Health, Inc. All rights reserved.