Journal Logo


1st International Conference on Natural Products for Cancer Prevention and Therapy 31 August–2 September 2015 Istanbul, Turkey


doi: 10.1097/CAD.0000000000000311
  • Free

The following abstract and its reference list was unintentionally omitted from this abstract supplement publication and is reproduced below 1.

Investigation of in-vitro and in-vivo anti-oxidant effects of Matricaria chamomilla extract on rat model of ulcerative colitis

M. Yunus Bektaya, Fatih Uçkayaa, Gulacti Topcua, Mukaddes Esrefoglub and Nihan Bayindirb

aPharmacy and bMedicine, Bezmialem Vakif University, Fatih, 34093 Istanbul, Turkey

Ulcerative colitis (UC) is a common, relapsing inflammatory bowel disease (IBD) presenting with ulcers and wounds located on the colonic intestinal mucosa. The difference between ulcerative colitis and Crohn’s disease is that ulcerative colitis is located on the colon and wounds are limited to the superficial mucosal membranes of intestinal lumen. The main pathological feature of disease is inflammatory destruction of inner layer of the colon lumen. The disease is clinically presented with rectal bleeding, abdominal pain and diarrhea. Even though there are different hypotheses about the aethiopathogenesis of disease, the exact causes of UC are currently unknown 1.

Inflammatory response and formation of cytokines result in an increase in radicals, reactive oxygen species (ROS) and reactive nitrogen species (RNS) in UC patients. The integrity of the mucosal membrane is erupted with enormous amounts of radicals, ROS, RNS, enzymes and cytokine formation 2–42–42–4. According to scientific evidence, in long term UC patients an increase in epithelial dysplasia and cancer complications has been noted 3. Secondary metabolites in Matricaria chamomilla (MC) have both anti-inflammatory and anti-oxidant radical scavenging properties and in our study, we investigated this plant’s protective effect on mucosal layer integrity 5.

In this study we examined the protective and complementary effects of MC. The grounded theory of choosing MC depends on anti-oxidant and anti-inflammatory effects of phenolic, flavonoidal secondary metabolites which are greatly found in herba of this plant 6.

Destruction of mucosal layer and induction of colitis and formation of inflammatory response was triggered by application of tri-nitro bezene sulphonic acid (TNBS) through the rectum. Methanol extract of drug was applied to UC-induced rats by oral gavage 7.

In-vitro examination of the anti-oxidant profile of methanol extract of MC has been evaluated by 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), 2,2-diphenyl-1-picrylhydrazyl free radical scavenging and CUPRAC methods 8,98,9. After administration of MC extracts, blood samples were drawn and analysis of total oxidative stress and total anti-oxidative stress assays was performed 10–1310–1310–1310–13. Histological investigation of bowel specimens has also been compared with a control group.


1. Conrad K, Roggenbuck D, Laass MW. Diagnosis and classification of ulcerative colitis. Autoimmun Rev 2014; 13:463–466.
2. Soobrattee MA, Neergheen VS, Luximon-Ramma A, Aruomab OI, Bahorun T. Phenolics as potential antioxidant therapeutic agents: mechanism and actions. Mutat Res 2005; 579:200–213.
3. Danese S, Fiocchi C. Ulcerative colitis. N Engl J Med 201; 365:1713–1725.
4. Esmaily H, Sanei Y, Abdollahi M. Autoantibodies and an immune-based rat model of inflammatory bowel disease. World J Gastroenterol 2013; 19:7569–7576.
5. Konga JM, Chiaa LS, Goha NK, Chiaa TF, Brouillardb R. Analysis and biological activities of anthocyanins. Phytochemistry 2003; 64:923–933.
6. Petruľová-Poracká V, Repčák M, Vilková M, Imrich J. Coumarins of Matricaria chamomilla L., Aglycones and glycosides. Food Chem 2013; 141:54–59.
7. Isik F. The protective effects of black cumin (Nigella Sativa) oil on TNBS induced experimental colitis in rats. Republic of Turkey: Marmara University Institute of Health Sciences; 2009.
8. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958; 181:1199–1200.
9. Apak R, Güçlü K, Özyürek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem 2004; 52:7970–7981.
10. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem 2004; 37:277–285.
11. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005; 38:1103–1111.
12. Aktepe N, Kocyigit A, Yukselten Y, Taskin A, Keskin C, Celik H. Increased DNA damage and oxidative stress among silver jewelry workers. Biol Trace Elem Res 2015; 164:185–191.
13. Selek S, Ozer OF, Kalinbacoglu C, Islek I, Islek T, Arpaci B, et al.. Paraoxonase-1 phenotype and its relationship with mean platelet volume and oxidative stress in coronary artery disease. Bezmialem Science 2015; 2:105.
Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.