Preclinical ReportsmiR-203a suppresses cell proliferation by targeting RING-finger protein 6 in colorectal cancerMiao, Jiyua,,*; Hou, Nia,,*; Yang, Wanwanb; Jiang, Qiuyua; Xue, Wanjuanc; Wang, Xiaofeid; Zhang, Huahuac; Xiong, Xiaofana; Wang, Lumina; Zhao, Lingyua; Huang, Chena,,dAuthor Information aDepartment of Cell Biology and Genetics, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center bDepartment of anesthesiology and Perioperative Medicine, Xijing Hospital, the Fourth Military Medical University, Xi’an cMedical College, Yan’an University, Yan’an dKey Laboratory of Environment and Genes Related to Diseases, Xi’an Jiaotong University Health Science Center, Xi’an, China * Jiyu Miao and Ni Hou contributed equally to the writing of this article. Received 24 July 2019 Revised form accepted 3 November 2019 Correspondence to Chen Huang, Department of Cell Biology and Genetics, School of Basic Medical Sciences/Key Laboratory of Environment and Genes Related to Diseases, Xi’an Jiaotong University, Ministry of Education of China, 76 Western Yanta Road, Xi’an 710061, Shaanxi, China, Tel/fax: +86 29 8265 5077; e-mail: firstname.lastname@example.org Anti-Cancer Drugs: July 2020 - Volume 31 - Issue 6 - p 583-591 doi: 10.1097/CAD.0000000000000874 Buy Metrics Abstract Colorectal cancer (CRC) is one of most common cancers worldwide. Although miR-203a is reported as a tumor suppressor involved in cell progression in some cancers, the role of miR-203a in CRC is still controversial and the underling mechanism of miR-203a in CRC remains unclear. Here, we demonstrated that low expression of miR-203a had poorer survival in CRC patients. miR-203a was down-regulated in most human colon cancer cells. Overexpression of miR-203a could inhibit colon cancer cell proliferation and arrest cell cycle in G1 phase. Bioinformatics and dual luciferase reporter assay confirmed that RING-finger protein 6 (RNF6) was a target gene of miR-203a. Silencing RNF6 inhibited cell proliferation and arrest cell cycle in G1 phase. RNF6 overexpression reversed the effects of miR-203a overexpression in colon cancer cells. Taken together, our data indicate that miR-203a inhibits colon cancer cell proliferation by targeting RNF6, offer novel insights into the regulatory network of miR-203a-modulated cell cycle and proliferation, and suggest that miR-203a a potential therapeutic target in CRC treatment. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.