Preclinical ReportsOverexpressed CXCR4 and CCR7 on the surface of NK92 cell have improved migration and anti-tumor activity in human colon tumor modelYang, Lilia,,*; Huang, Changxina,,*; Wang, Congjieb; Zhang, Siyub; Li, Zhaoyangc; Zhu, Yingd; Li, Dae; Gao, Lanlanf; Ge, Zuanming; Su, Mengh; Feng, Xii; Li, YongqiangjAuthor Information aThe Affiliated Hospital of Hangzhou Normal University bHangzhou Normal University School of Medicine, Hangzhou cThe Affiliated Hospital of Hangzhou Normal University dZhejiang Provincial Hospital of Traditional Chinese Medicine eSir Run Run Shaw Hospital, Zhejiang University School of Medicine fHangzhou Normal University gHangzhou Normal University hZhejiang University of Traditional Chinese Medicine iHangzhou Normal University jThe Affiliated Hospital of Hangzhou Normal University, Zhejiang Province, China * Dr. Lili Yang and Dr. Changxin Huang contributed equally to the writing of this article. Received 29 January 2019 Revised form accepted 25 October 2019 Supplemental Digital Content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's website, www.anti-cancerdrugs.com. Correspondence to Yongqiang Li, Hangzhou Normal University School of Medicine, No. 16 Xuelin Street, Xiasha Higher Education Park, Hangzhou, 310015 Zhejiang Province, China, Tel: +15990199891; fax: +0571 88021730; e-mail: email@example.com Anti-Cancer Drugs: April 2020 - Volume 31 - Issue 4 - p 333-344 doi: 10.1097/CAD.0000000000000868 Buy SDC Metrics Abstract Successive infusion of natural killer cells is increasingly being explored as a treatment for cancer patients. The inadequate homing of natural killer cells into the tumor site resulted in the poor efficacy of natural killer cells on solid tumors. For the adoptive transfer of tumor-directed natural killer cell has been proved effective, it is hypothesized that there must be more association between the tumor-produced chemokines and the natural killer cells-expressed chemokine receptors. Increased CXCL12 and CCL21 could ameliorated colorectal cancer via generating an anti-tumor environment by preferentially attracting natural killer cells which expressed the chemokine receptor CXCR4 and CCR7. This study demonstrated that overexpressed CXCR4 and CCR7 on the surface of NK92 cell enhanced their migration to human colon cells. Moreover, the administration of such natural killer cells resulted in tumor shrinkage and a significantly increased survival of experimental mice when compared to ones undergoing the treatment of xenografts with natural killer cells expressing only the mock control. These suggested that chemokine receptor engineered natural killer cells could be a promising tool to improve adoptive tumor immunotherapy. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.