Preclinical ReportsCombination treatment of bicalutamide and curcumin has a strong therapeutic effect on androgen receptor-positive triple-negative breast cancersDong, Shenglia; Alahari, Suresh K.a,,bAuthor Information aDepartment of Biochemistry and Molecular Biology bStanley S. Scott Cancer Center, LSUHSC School of Medicine, New Orleans, Louisiana Received 2 May 2019 Revised form accepted 22 November 2019 Supplemental Digital Content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's website, www.anti-cancerdrugs.com. Correspondence to Suresh K. Alahari, PhD, CSRB 406, LSUHSC, New Orleans, LA 70112, USA, Tel: +504 568 4734; e-mail: firstname.lastname@example.org Anti-Cancer Drugs: April 2020 - Volume 31 - Issue 4 - p 359-367 doi: 10.1097/CAD.0000000000000880 Buy SDC Metrics Abstract Triple-negative breast cancers account for approximately 15–20% of breast cancer patients. Due to lack of expression of estrogen receptor, PR and human epidermal growth factor receptor 2 in triple-negative breast cancers, there are no targeted therapies available for these cancers. Therefore, a major research priority is to find potential therapeutic targets. Androgen receptor is present in 80–90% of all breast cancers, including 55% of estrogen receptor-α–negative cancers and 12%–35% of triple-negative breast cancers. Androgen receptor stimulates growth and survival in triple-negative breast cancer cells. Treatment with bicalutamide, an androgen receptor antagonist, has a good benefit for AR+ triple-negative breast cancer patients. AR+ triple-negative breast cancer cells were treated with curcumin or bicalutamide alone or in combination of both together. Cell growth, apoptosis and Wnt signaling pathways were examined. We found that curcumin dramatically suppressed Wnt signaling pathway in AR+ triple-negative breast cancer cells. Curcumin treatment inhibited androgen receptor protein expression in AR+ triple-negative breast cancer cells. Combination treatment of curcumin and bicalutamide has a robust increase in apoptosis. Furthermore, the combination treatment suppressed the growth of AR+ triple-negative breast cancer cells more effectively than with the single drug alone. Our data indicate that androgen receptor inhibition is a potential therapeutic approach for AR+ triple-negative breast cancers. In summary, our study for the first time shows that the combination treatment of curcumin and bicalutamide is effective in AR+ triple-negative breast cancer cells. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.