Preclinical ReportsA dual cancer-specific recombinant adenovirus suppresses the growth of liver cancer cells in vivo and in vitroTian, Yufeia,,b; Yao, Weic; He, Dongyund; Xu, Yingyingd; Li, Yiquana,,b; Zhu, Yilonga,,b; Fang, Jinbob; Bai, Bingb; Li, Xiaoa,,b,,e; Sun, Lilia,,f; Jin, Ningyia,,b,,eAuthor Information aInstitute of Military Veterinary Medicine, Academy of Military Medical Science bChangchun University of Chinese Medicine, Changchun cCenter for Disease Control and Prevention, Agency for Offices Administration, Central Military Commission, Beijing dChina-Japan Union Hospital of Jilin University, Changchun, Jilin eJiang su Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou fDepartment of Head and Neck Surgery, Tumor Hospital of Jilin Province, Changchun, China Received 29 June 2019 Revised form accepted 24 September 2019 Correspondence to Ningyi Jin, PhD, Institute of Military Veterinary Medicine, Academy of Military Medical Science, Liuying west road, 666, Jingyue Economic and Technological Development Zone, Changchun, Jilin 130122, China, Tel: +86 0431 86985923; fax: +86 0431 87985861; e-mail: firstname.lastname@example.org Anti-Cancer Drugs: February 2020 - Volume 31 - Issue 2 - p 110-122 doi: 10.1097/CAD.0000000000000854 Buy Metrics Abstract Oncolytic virus therapy is emerging as important means in cancer treatment. In a previous study, we constructed a dual cancer-specific antitumor recombinant adenovirus, designating it Ad-apoptin-hTERTp-E1a (Ad-VT). This study aimed to investigate the anticancer potential of recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) in liver cancer. Crystal Violet staining and CCK-8 assays were used to analyse the inhibitory effect of recombinant adenovirus on human hepatoma cell line QGY-7703 and SMMC-7721. Ad-VT had a significant tumour killing inhibitory effect on QGY-7703 and SMMC-7721 cells that was both dose and a time dependent. Ad-VT-induced apoptosis of QGY-7703 cells was detected using Hoechst, Annexin V, and JC-1 staining, as well as western blotting. Recombinant adenovirus had a strong apoptosis-inducing effect on QGY-7703 cells, and killed QGY-7703 cells mainly through the mitochondrial apoptotic pathway. QGY-7703 cells invasion were detected using cell-scratch and Transwell assays. Recombinant adenovirus could significantly inhibit the invasion of QGY-7703 cells over a short period of time. The pGL4.51 plasmid was used to transfect QGY-7703 cells to construct tumour cells stably expressing luciferase (QGY-7703-LUC). The tumour inhibition effect of Ad-VT in vivo was subsequently confirmed by establishing a tumour-bearing nude mouse model. Ad-VT could effectively inhibit tumour growth and prolong survival of the mice. Recombinant adenovirus Ad-VT has the characteristics of tumour-specific replication and specific tumour killing, and could inhibit the growth of liver cancer QGY-7703 cells and promote their apoptosis. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.