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Synergistic anti-tumour effects of Clostridium butyricum in combination with apatinib in CT26 colorectal tumour-bearing mice

Xin, Menga,*; Xie, Qib,*; Ma, Lina; An, Gec; Xiao, Junjuana; Li, Junweia; Liu, Xiaolina; Gao, Pengb; Liang, Jinga; Li, Yana

doi: 10.1097/CAD.0000000000000817
Preclinical Reports
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To investigate the synergistic antitumour effect of Clostridium butyricum combined with apatinib on colorectal cancer in mice. Murine colorectal carcinoma cell line CT26.WT cells were xenografted into the skin of BALB/c mice. Tumour-bearing mice were randomly divided into four groups, and given different treatment options (PBS control; C. butyricum; apatinib; C. butyricum + apatinib). Real-time PCR was used to detect C. butyricum content in the intestine of mice given C. butyricum. The effects of various regimens on tumour growth were monitored, and CD31, proliferating cell nuclear antigen (PCNA), Bcl-2 and cleaved caspase-3 expressions in tumour were analysed by immunohistochemistry. C. butyricum combined with apatinib significantly inhibits tumour growth with decreased CD31, PCNA and Bcl-2 expressions, and increased cleaved caspase-3 expressions. Our study confirms that C. butyricum combined with apatinib in the treatment of xenografted colon tumour in mice can significantly inhibit tumour growth and promote cell apoptosis than apatinib alone treatments, providing the reference for clinical treatments.

aDepartment of Oncology, Shandong Provincial Qianfoshan Hospital, Shandong University

bLaboratory of Microvascular Medicine, Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University

cMedical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, P.R. China

* Dr. Meng Xin and Dr. Qi Xie contributed equally to the writing of this article.

Received 18 December 2018 Revised form accepted 17 May 2019

Correspondence to Dr. Yan Li, Department of Oncology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, P.R. China, Tel/fax: +86 0531 89269516; e-mail: liyan16766@163.com

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