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Evaluation of the antileukemic effects of neurokinin-1 receptor antagonists, aprepitant, and L-733,060, in chronic and acute myeloid leukemic cells

Dikmen, Mirişa; Gökhaner, Gökhanc; Cantürk, Zerrinb

doi: 10.1097/CAD.0000000000000769
Preclinical Reports
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Neurokinin-1 receptor (NK1R) antagonists are known for their anxiolytic, antiemetic, anticancer, and anti-inflammatory effects. Aprepitant is used in vomiting and nausea, which are the most common side-effects of patients undergoing chemotherapy for cancer. L-733,060 has been shown to have anxiolytic and antidepressant effects in animal studies and anticancer effect in in-vitro studies. Previous anticancer activity studies with NK1R antagonists have reported that NK-1 antagonists have an antitumoral activity on gastric carcinoma, larynx carcinoma, retinoblastoma, hepatocarcinoma, glioma, neuroblastoma, and osteoblastoma cells. In this study, we have aimed to show and compare the antileukemic effects of aprepitant and L-733,060 on acute and chronic myeloid leukemic cells by using in-vitro experiments, such as WST-1, cell imaging, annexin-V binding, soft agar colony formation, and Hoescht staining. As a result, we have determined that both aprepitant and L-733,060 had strong antiproliferative effects on K562 and HL-60 cells. Moreover, the two drugs caused significant apoptosis and decreased colony forming depending on concentration increase. These findings suggested that NK1R antagonists exhibited antileukemic activities and may be considered to have a novel therapeutic potential for acute and chronic myeloid leukemia.

Departments of aPharmacology

bPharmaceutical Microbiology, Faculty of Pharmacy

cDepartment of Pharmacology, Graduate School of Health Sciences, Anadolu University, Eskisehir, Turkey

Received 7 October 2018 Revised form accepted 29 January 2019

Correspondence to Miriş Dikmen, PhD, Department of Pharmacology, Faculty of Pharmacy, Anadolu Universty, Eskisehir, Centrum TR-26470, Turkey Tel: + 90 222 335 0580 x3748; fax: + 90 222 335 0750; e-mail: mirisd@anadolu.edu.tr

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