Identification of oncogenic long noncoding RNAs CASC9 and LINC00152 in oral carcinoma through genome-wide comprehensive analysisChen, Mingwei*; Xu, Xiaonan*; Ma, HaiqingAnti-Cancer Drugs: April 2019 - Volume 30 - Issue 4 - p 356–362 doi: 10.1097/CAD.0000000000000725 PRECLINICAL REPORTS Buy SDC Abstract Author InformationAuthors Article MetricsMetrics Oral carcinoma (OC) is the major cancer type in the head and neck region; however, the molecular mechanisms of its pathogenesis and progression remain poorly understood. In recent years, the long noncoding RNAs (lncRNAs) have been uncovered as critical regulators in the development and progression of multiple human cancers, but most of the lncRNAs expression patterns, clinical relevance, and biological functions in OC are still unclear. To better understand the significance of lncRNAs in OC carcinogenesis, we analyzed the expression levels of lncRNAs between OC and healthy oral mucosa using The Cancer Genome Atlas Cancer Genome RNA sequencing data and another three independent microarray datasets from Gene Expression Omnibus. As a result, we found that thousands of lncRNAs expression are dysregulated in OC, and further somatic copy number variation analyses showed that some of these lncRNAs alterations are associated with copy number amplification or loss in OC. Moreover, lots of lncRNAs expression levels are associated with OC patients’ overall survival and recurrence-free survival; for example, higher CASC9, LINC01232, and MIR4435-1HG expression levels are related to shorter overall survival and recurrence-free survival in OC patients. Finally, the potential function of two lncRNAs (CASC9 and LINC00152) that were upregulated in OC tissues and associated with patients’ survival time was verified by loss-of-function assays in OC cells. Our findings indicate that these altered lncRNAs might play important roles in the development of OC, and our data can provide a valuable list of lncRNAs candidates for further investigation of their function and mechanisms in OC. Department of Stomatology, Jining First People’s Hospital, Jining, People’s Republic of China * Mingwei Chen and Xiaonan Xu contributed equally to the writing of this article. Correspondence to Haiqing Ma, MSc, Department of Stomatology, Jining NO.1 People’s Hospital, Jining 272011, People’s Republic of China Tel/fax: +86 053 7225 3294; e-mail: firstname.lastname@example.org Received May 20, 2018 Accepted November 8, 2018 Copyright © 2019 Wolters Kluwer Health, Inc. All rights reserved.