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Role of p38 MAPK activation and mitochondrial cytochrome-c release in allicin-induced apoptosis in SK-N-SH cells

Zhuang, Jianhui; Li, Yu; Chi, Yufen

doi: 10.1097/CAD.0000000000000340
PRECLINICAL REPORTS
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Here, we investigate the apoptotic effect of allicin, the predominant component of freshly crushed garlic, on neuroblastoma cells. In this paper, the authors have first assessed the effect of allicin on human neuroblastoma SK-N-SH cells and then investigated the underlying mechanism. The results indicate that allicin suppresses SK-N-SH cell growth in a dose-dependent and time-dependent manner and that 5 μmol/l of allicin leads to a significant increase in apoptotic rate with annexin-V/PI double staining. Western blot analysis shows that treatment with allicin-induced apoptosis through activation of caspases-3 and 9. Phosphorylation of p38 MAPK contributes to allicin-induced apoptosis upstream of caspase activation. Using p38 MAPK inhibitor, the authors discovered that p38 MAPK activation subsequently induces the release of cytochrome-c from mitochondria into the cytosol. Taken together, the results demonstrate that allicin can activate the p38 MAPK pathway, which leads to mitochondrial release of cytochrome-c, thus inducing SK-N-SH cell apoptosis. Overall, this study suggests that allicin may be used as one of the novel pharmacological treatment strategies in neuroblastoma.

Departments of aUrinary Surgery

bGynaecology and Obstetrics

cPaediatrics, People’s Hospital of Rizhao, Rizhao, Shandong, China

Correspondence to Yufen Chi, BSc, Department of Paediatrics, People’s Hospital of Rizhao, Rizhao 276826, Shandong Province, China Tel/fax: +86 633 338 8258; e-mail: 269021239@qq.com

Received May 27, 2015

Accepted December 21, 2015

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