Transplantation Surgery and ResearchChyloid Fat Carried Adipose-Derived Mesenchymal Stem Cells Accelerate Wound Healing Via Promoting AngiogenesisGeng, Yinana,b; Yang, Jinxiub; Li, Shiyia,b; Chen, Minliang PhDa,bAuthor Information From the aMedical School of Chinese People's Liberation Army of China bDepartment of Burn and Plastic Surgery, The Fourth Medical Centre, Chinese People's Liberation Army of China General Hospital, Beijing, People's Republic of China. Received October 19, 2020, and accepted for publication, after revision December 31, 2020. Availability of data and materials: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request. Ethics approval: This study has been proved by the Ethics Committee of Chinese People's Liberation Army of China (PLA) General Hospital. Conflicts of interest and sources of funding: This work is financially supported by the Key Projects of the Military Logistics Research Plan (BWS14J047) and the Military Medical Innovation Research Project of PLA General Hospital (CX19012). Reprints: Minliang Chen, PhD, Department of Burn and Plastic Surgery, the Fourth Medical Centre, Chinese PLA General Hospital, 51 Fucheng Rd, Haidian District, Beijing, People's Republic of China 100048. E-mail: [email protected]. Annals of Plastic Surgery: October 2021 - Volume 87 - Issue 4 - p 472-477 doi: 10.1097/SAP.0000000000002778 Buy Metrics Abstract Impaired wound healing is responsible for significant morbidity and mortality worldwide. It is necessary to find a stable, efficient, and safe method to promote soft tissue wound healing. Fat grafting has become increasingly popular in contouring procedures. However, more recently, there has been an emphasis on its regenerative potential. In this study, we established the wound healing model using nude mice. Hematoxylin and eosin and Masson stainings were performed to assess the effect of chyloid fat on the histology of wound healing. A laser Doppler perfusion imager was used to evaluate the blood perfusion of wounds. Immunohistochemistry was carried out to detect the expression of CD31 in wound tissues. The results suggested that after treatment with granule fat or chyloid fat, wound healing was accelerated and blood perfusion was promoted. In addition, granule fat or chyloid fat treatment promoted the angiogenesis of the wound. In addition, we evaluated the amount of adipose-derived mesenchymal stem cells in chyloid fat and granule fat. It was found that chyloid fat contained more adipose-derived mesenchymal stem cells than granule fat did. In conclusion, we proved that chyloid fat could significantly accelerate the wound healing process via promoting angiogenesis. The adipose-derived mesenchymal stem cell plays a critical role in this effect of chyloid fat. Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.