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The Effect of Chyle Fat Injection on Human Hypertrophic Scars in an Animal Model

A New Strategy for the Treatment of Hypertrophic Scars

Chen, Junnan, MD*†‡; Lai, Linying, MB; Ma, Kui, MM; Xu, Xiao, MD; Huang, Zhenya, MB*†; Zhou, Guiwen, MB; Zhou, Yunchao, MM; Liang, Liming, MD; Chen, Minliang, MD*†

doi: 10.1097/SAP.0000000000001784
Burn Surgery and Research
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Background Chyle fat transplantation has shown positive effects on preexisting human hypertrophic scars (HSs) in a nude mouse HS graft model.

Methods Hypertrophic scar fragments were obtained from 5 surgically treated burn patients and implanted into the backs of nude mice in 3 groups: group A, control; group B, triamcinolone; and group C, chyle fat. The specimens were implanted after the corresponding intralesional injection in each group, and the mice were observed for 4 weeks. In total, 18 mice and 72 scar specimens were studied. After 4 weeks, the HSs were removed from the mice. Then, the scar weights, histology, and decorin staining were assessed to evaluate the therapeutic efficacy.

Results An obviously significant difference was observed in the HS weight reduction between groups A and C (P < 0.01), and a significant difference in the HS weight reduction was observed between groups A and B (P < 0.05). However, there was no significant difference between groups B and C. The treatment groups (groups B and C) showed strong decorin staining. Furthermore, the decorin staining was much stronger in group C than in group B (P < 0.05). Significant differences in extracellular matrix deposition were observed among the 3 groups, as determined by Masson trichrome staining. Both groups B and C showed significant therapeutic efficacy compared with group A, and group C exhibited a significant therapeutic effect compared with group B (P < 0.05).

Conclusions This study indicates that chyle fat grafting is beneficial for treating HSs.

From the *Medical School of Chinese PLA;

Chinese PLA General Hospital; and

The General Hospital of the PLA Rocket Force, Beijing, People’s Republic of China.

Received August 14, 2018, and accepted for publication, after revision November 4, 2018.

Conflicts of interest and sources of funding: This study was supported by the Chinese National Nature Science Fund (no. 81772085). The authors declare no conflict of interest.

Reprints: Minliang Chen, MD, Department of Burn and Plastic Surgery, the Fourth Medical Centre, Chinese PLA General Hospital, 51 Fucheng Rd, Haidian District, Beijing, People's Republic of China 100048. E-mail: chenml@sohu.com.

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