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The Potential of Acellular Dermal Matrix Combined With Neural Stem Cells Induced From Human Adipose-Derived Stem Cells in Nerve Tissue Engineering

Syu, Wei-Ze, PhD*; Chen, Shyi-Gen, MD, MPH; Chan, James Yi-Hsin, MD, PhD*‡; Wang, Chih-Hsin, MD; Dai, Niann-Tzyy, MD, PhD; Huang, Shih-Ming, PhD§

doi: 10.1097/SAP.0000000000001731
Peripheral Nerve Surgery and Research

Introduction Reconstruction of segmental peripheral nerve gap is still challenging when the autografts are unavailable owing to limited availability of donor site and functional recovery. The creation of artificial conduits composed of biological or synthetic materials is still developing. Acellular dermal matrix (ADM) has been widely studied and its extension and plasticity properties may become suitable nerve conduits under different forms of nerve gaps. Adipose-derived stem cells (ADSCs) have the potential to differentiate into various cell types of different germ layers including neural stem cells (NSCs). The purpose of this experiment is to use ADM as a scaffold combined with NSCs induced by ADSCs to establish neural tissue engineering.

Methods The ADSCs were isolated from syringe-liposuction adipose tissue harvested from abdominal fat and then cultured in keratinocyte serum free media to trigger into neural stem cells. Stem cells were confirmed by the expression of surface markers nestin and SOX2 in NSCs with Western blot and immunofluorescent staining. Matrix enzyme treatment was used to obtain ADM to remove immunogenic cells while maintaining the integrity of the basement membrane complex and the extracellular matrix structure of the dermis. The NSCs were cocultured with ADM for 3 days, and survival markers Ki67 and neural stem cell markers nestin were detected.

Results These NSCs can form neurospheres and express nestin and SOX2. The NSC can further coculture with ADM, and it will continue to express survivor markers and neural stem cell markers on ADM.

Conclusions These findings provide evidence that the combination of ADM and NSC has the same potential as neural tissue engineering as other acellular sciatic nerve.

From the *Graduate Institute of Life Sciences;

Division of Plastic and Reconstructive Surgery, Tri-Service General Hospital;

Graduate Institute of Medical Sciences; and

§Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan.

Received September 25, 2018, and accepted for publication, after revision October 5, 2018.

Conflicts of interest and sources of funding: The work was supported in part by MOST106-2314-B-016-025, a grant from the Ministry of Science and Technology, Taiwan, and in part by TSGH-C107-118, a grant from the Tri-Service General Hospital, Taiwan. The authors declare no conflict of interest.

Reprints: Shyi-Gen Chen, MD, MPH, Division of Plastic and Reconstructive Surgery, Tri-Service General Hospital, National Defense Medical Center, 325, Sec. 2, Cheng-Kung Rd, Taipei 114, Taiwan. E-mail:

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