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Correlation Between IL-20 and De Quervain's Disease Severity

Kuo, Yao-Lung MD, PhD*; Jou, I-Ming MD, PhD; Wang, Ping-Hui MD; Yang, Tai-Hua MD§; Shen, Ching-Ju MD; Hsu, Che-Chia MD§

doi: 10.1097/SAP.0000000000001701
Research
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De Quervain's disease is a stenosing tenosynovitis of the first dorsal compartment of the wrist. Histopathological studies have reported that the thickening of the first dorsal retinaculum is characterized by degeneration rather than inflammation. However, significant infiltration of mast cells and macrophages was noted in a torn tendon study, which suggested that innate immune pathways are part of the mechanism that mediates early tendinopathy. Recently, Interleukin-20 (IL-20) has been reported to provoke potent inflammation and regulate angiogenesis and chemotaxis, which are important for the pathogenesis of inflammatory diseases. The main purpose of our study was to investigate the correlation between IL-20 and tumor necrosis factor (TNF-α) and clarify the potential predictor of tendinopathy progression. Hematoxylin and eosin (H & E) and immunohistochemistry (IHC) staining were used to score and analyze the clinical outcome. TNF-α, IL-20 and related inflammation cytokines were examined. Moreover, the tenocytes were cultured with a stimulator and were used to examine inflammatory cytokine secretions. A real-time polymerase chain reaction (Real-time PCR) was used to detect the gene expression profile. The IHC data showed that TNF-α is up-regulated in grade III de Quervain's. The analysis data showed that IL-20 is positively correlated with TNF-α and disease severity. The real-time PCR showed that the inflammation stimulator enhanced the expression of IL-20 mRNA expression. Inflammation cytokines such as TNF-alpha, transforming growth factor-β (TGF-β) and IL-1 have been used as predictors of de Quervain's; IL-20 is a new predictor based on this study. In the future, IL-20 expression's involvement in the molecular mechanism of the severity of de Quervain's should be further investigated.

From the *Department of Surgery, National Cheng Kung University, College of Medicine, Department of Surgery, National Cheng Kung University Hospital, Tainan and Dou-Liou Branch, Taiwan;

Department of Orthopedics, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan;

Department of Orthopedics, Chi-Mei Medical Center, Tainan, Taiwan;

§Department of Orthopedics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; and

Department of Gynecology and Obstetrics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.

Received September 12, 2018, and accepted for publication, after revision September 21, 2018.

Supported in part by Kaohsiung Medical University Grant, grant No. KMU-106 T11.

Conflicts of interest and sources of funding: none declared.

Reprints: Ching-Ju Shen, MD, Department of Gynecology and Obstetrics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan. E-mail: chenmed.tw@yahoo.com.tw; Che-Chia Hsu, MD, Department of Orthopedics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 138 Sheng Li Rd, Tainan 704, Taiwan. E-mail: hcc918@mail.ncku.edu.tw.

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