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Gene Expression of Neurotrophins and Their Receptors in Keloids

Petecof, Rafael de Moraes, MS; Gragnani, Alfredo, MD, PhD; Isoldi, Felipe Contoli, MD, MS; Munoz, Gibrán Elias Harcha, MD; de Noronha, Samuel Marcos Ribeiro, PhD; Corrêa, Silvana Aparecida Alves, PhD; Ferreira, Lydia Masako, MD, PhD

doi: 10.1097/SAP.0000000000001648
Burn Surgery and Research

The aim of this study was to assess gene expression of neurotrophins and their receptors in keloids. Skin samples of normal skin and keloids were obtained from patients in the control (n = 12) and keloid (n = 12) groups, respectively. Ribonucleic acid was extracted from the skin specimens, purified, evaluated by spectrophotometry, and used to synthesize complementary DNA. Real-time quantitative polymerase chain reaction analysis of 84 human neurotrophin genes and their receptors was performed. Twelve genes, including heat shock 27-kDa protein 1, gastrin-releasing peptide receptor, corticotropin-releasing hormone receptor 2, neuropeptide Y Y2 receptor, interleukin 6 signal transducer, nerve growth factor, metallothionein 3, B-cell chronic lymphocytic leukemia/lymphoma 2, cholecystokinin A receptor, persephin, galanin receptor 2, and fibroblast growth factor receptor 3, were down-regulated in keloid tissue compared with normal skin. The genes 27-kDa heat shock protein 1, gastrin-releasing peptide receptor, corticotropin-releasing hormone receptor 2, nerve growth factor, metallothionein 3, B-cell chronic lymphocytic leukemia/lymphoma 2, and persephin protein were considered priority genes associated with keloid formation.

From the Division of Plastic Surgery, Department of Surgery, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.

Received February 5, 2018, and accepted for publication, after revision August 9, 2018.

Conflicts of interest and sources of funding: none declared. Indirect, partial financial support for this study was provided by the Research Support Foundation of the State of São Paulo (FAPESP). This independent public foundation had no roles in the design and conduct of the study, including collection, management, analysis, and interpretation of data and preparation, review, and approval of the article.

All authors have made substantial contributions to the manuscript and take public responsibility for the content.

Reprints: Alfredo Gragnani, MD, PhD, Division of Plastic Surgery, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Pedro de Toledo 720, Edifício Jairo Ramos, 2° andar; 04039-002 São Paulo, São Paulo, Brazil. E-mail: agragnanif@gmail.com.

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