To evaluate the anastomotic potential of prevascular tissue constructs generated from scaffold-free self-assembly of human endothelial and fibroblast cells, tissue constructs were implanted into athymic mice and immune-competent rats. Analysis of xenografts placed into hind limb muscle defects showed vascular anastomotic activity by 3 days after implantation and persisting for 2 weeks. Integration of the implanted prevascular tissue constructs with the host circulatory system was evident from presence of red blood cells in the implant as early as 3 days after implantation. Additionally, analysis of 3-day xenografts in the rat model showed activation of skeletal muscle satellite cells based on Pax-7 and MyoD expressions. We conclude that prevascular tissue constructs generated from scaffold-free self-assembly of human endothelial and fibroblast cells are a promising tool to provide both vascular supply and satellite cell activation toward the resolution of skeletal muscle injury.
From the *Department of Regenerative Medicine and Cell Biology, †Department of Surgery, Medical University of South Carolina, Charleston, SC.
Received August 22, 2014, and accepted for publication, after revision, December 12, 2014.
Michael J. Yost and Christopher J. Drake share cosenior authorship.
Conflicts of interest: none declared.
Sources of funding: NIH R01 DE019355 (MJY), NIH R01 HL080168 (CJD), and NSF EPSCoR RII EPS-090375 (CJD).
Reprints: Micahel J. Yost, PhD, Department of Surgery, 173 Ashley Ave., CRI 605, MSC 508 Charleston, SC 29425-0508. E-mail: email@example.com.