Experimental PapersAdipose-Derived Stromal Cells Accelerate Wound Healing in an Organotypic Raft Culture ModelCollawn, Sherry S. MD, PhD; Banerjee, N. Sanjib PhD; de la Torre, Jorge MD; Vasconez, Luis MD; Chow, Louise T. PhD Author Information From the *Division of Plastic Surgery, University of Alabama, Birmingham, AL; and †Department of Biochemistry and Molecular Genetics, University of Alabama, Birmingham, AL. Received October 2, 2011, and accepted for publication, after revision, October 4, 2011. Conflicts of interest and sources of funding: This work was funded by NIH NCI grant CA83679 (L.T.C.). The authors have no conflicts of interest to declare. Presented at the SESPRS meeting Naples, Florida, June 8, 2011. SESPRS funding to continue this research was presented at this meeting. Reprints: Sherry Stine Collawn, MD, PhD, University of Alabama, Birmingham, Birmingham, Alabama, 510 20th Street South, FOT 1102, Birmingham, AL 35294. E-mail: [email protected]. Annals of Plastic Surgery: May 2012 - Volume 68 - Issue 5 - p 501-504 doi: 10.1097/SAP.0b013e31823b69fc Buy Metrics Abstract Adipose tissue is a known reservoir of multipotent mesenchymal stem cells, which can be manipulated in culture to produce cells with different phenotypes. The goal of this study was to determine whether the addition of these multipotential cells to organotypic, human skin equivalent cultures would accelerate wound healing after laser injury. For our initial studies, we were able to obtain 3-dimensional raft cultures from adult skin explanted directly onto the dermal equivalent containing human fibroblasts with or without adipose-derived stromal cells (ADSCs). Two days after laser injury, the raft cultures of skin explants that contained ADSCs had a completely healed multilayered epidermis, whereas the control raft culture without the adipose-derived cells still had areas of injury. With this encouraging outcome, these experiments were then repeated in a raft culture system initiated from dissociated primary adult human keratinocytes on the humanized dermal equivalent. Again, the cultures containing ADSCs healed faster than the control cultures. In conclusion, these data provide support to our hypothesis that ADSCs are an excellent and readily available source of factors necessary for accelerated wound healing and tissue regeneration. Copyright © 2012 Wolters Kluwer Health, Inc. All rights reserved.