Southeastern Society of Plastic and Reconstructive SurgeonsCellular Incorporation Into Electrospun Nanofibers: Retained Viability, Proliferation, and Function in Fibroblastsvan Aalst, John A. MD*; Reed, Courtney R. BS*; Han, Li PhD†; Andrady, Tony PhD†; Hromadka, Michael BS‡; Bernacki, Susan PhD§; Kolappa, Kamalkumar BS‡; Collins, James B. BS‡; Loboa, Elizabeth G. PhD§Author Information From the *Division of Plastic and Reconstructive Surgery, University of North Carolina, Chapel Hill, NC; †Research Triangle Institute International, Research Triangle Park, NC; ‡University of North Carolina School of Medicine; and §Joint Department of Biomedical Engineering at UNC-Chapel Hill and NC State University, Raleigh, NC. Received September 11, 2007, and accepted for publication January 6, 2008. Supported by the David W. Furnas award, granted by the American Association of Plastic Surgeons (to J.A.v.A.). Reprints: John A. van Aalst, MD, Division of Plastic and Reconstructive Surgery, Department of Surgery, University of North Carolina, Chapel Hill, 7030 Burnett-Womack, CB 7195, Chapel Hill, NC 27514. E-mail: email@example.com. Annals of Plastic Surgery: May 2008 - Volume 60 - Issue 5 - p 577-583 doi: 10.1097/SAP.0b013e318168db3e Buy Metrics Abstract Nanofibers are an emerging scaffold for tissue engineering. To date no one has reported cell incorporation into nanofibers. Human foreskin fibroblasts and human adipose-derived adult stem cells (hADAS) were grown to confluence, resuspended in phosphate-buffered saline, and then solubilized in polyvinyl alcohol (PVA). Nanofibers were created using an electrospinning technique across an electric potential of 20 kV. Cell interaction with nanofibers was assessed with optical microscopic imaging and scanning electron microscopy. PVA nanofibers with incorporated cells were then solubilized in phosphate-buffered saline; cell viability was assessed by trypan blue exclusion. Viable cells were allowed to proliferate. Chondrogenesis in fibroblasts was induced with TGF-β1. Both fibroblasts and hADAS survived the electrospinning process and were incorporated into PVA nanofibers. hADAS cell proliferation was negligible; however, fibroblasts proliferated and showed retained ability to undergo chondrogenesis. Cells can be incorporated into nanofibers, with maintained viability, proliferation, and function. © 2008 Lippincott Williams & Wilkins, Inc.