Original ArticleNovel Lymphography Using Indocyanine Green Dye for Near-Infrared Fluorescence LabelingOgata, Fusa MD*; Azuma, Ryuichi MD†; Kikuchi, Makoto PhD*; Koshima, Isao MD‡; Morimoto, Yuji MD, PhD§Author Information From the *Departments of Medical Engineering, †Plastic Surgery, and ‡Integrative Physiology and Bio-Nano Medicine, National Defense Medical College, Saitama, Japan; and §Plastic and Reconstructive Surgery, Graduate School of Medicine, University of Tokyo, Tokyo, Japan. Received June 3, 2006, and accepted for publication, after revision, September 25, 2006. Reprints: Fusa Ogata, MD, Department of Medical Engineering, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan. E-mail: [email protected]. Annals of Plastic Surgery: June 2007 - Volume 58 - Issue 6 - p 652-655 doi: 10.1097/01.sap.0000250896.42800.a2 Buy Metrics AbstractIn Brief Lymphedema is known to be caused by many pathologic conditions; however, its diagnostic and therapeutic strategies remain to be unestablished. In this study, we investigated the usefulness of a novel lymphographic method based on fluorometric sensing using indocyanine green (ICG) dye for imaging lymphatic vessels using rat models. The real-time imaging system enabled visualization of superficial lymphatic vessels with a diameter of 0.1 mm in 33 frames/second. In addition, morphologic changes in lymphatic vessels in a radiation-induced lymphedema model were detected even at the latent stage. These results suggest that this imaging technique is acceptable as an evaluation method for the lymphatic system. In a rat model, lymphangiography was successfully performed using fluorometric testing with indocyanine green dye, enabling visualization of vessels as small as 0.1 mm. © 2007 Lippincott Williams & Wilkins, Inc.