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May 2021 A&A Video Summary: Featured Article 1

Video Author: Lance Lichtor
Published on: 04.22.2021
Associated with: May 2021, Volume 132, Issue 5;

Video summary of the featured article “Sleep Loss in the Hospitalized Patient and Its Influence on Recovery From Illness and Operation” in the May 2021 issue of Anesthesia & Analgesia.

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Supplemental Video 1A: Unstimulated control.
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Human neutrophils were isolated and seeded onto coated µ-slides (ibidi) and stimulated with 100 nM PMA. Recordings were performed for 6 hours every 2 minutes in 5% CO2 at 37°C. Blue: Hoechst-Dye (cell core); Orange: Sytox (extracellular DNA of impermeable cells and intracellular DNA once membrane is permeable); brightfield for cell visualization.
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Supplemental Video 1B: PMA-stimulated sample.
Creator: Andreas Margraf, Nadine Ludwig, Alexander Zarbock, Jan Rossaint
Duration: 00:18
Human neutrophils were isolated and seeded onto coated µ-slides (ibidi) and stimulated with 100 nM PMA. Recordings were performed for 6 hours every 2 minutes in 5% CO2 at 37°C. Blue: Hoechst-Dye (cell core); Orange: Sytox (extracellular DNA of impermeable cells and intracellular DNA once membrane is permeable); brightfield for cell visualization.
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